Fang H H P, Liang D W, Zhang T, Liu Y
Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
Water Res. 2006 Feb;40(3):427-34. doi: 10.1016/j.watres.2005.11.025. Epub 2006 Jan 9.
Over 99% of phenol was effectively degraded in an upflow anaerobic sludge blanket (UASB) reactor at 55 degrees C with 40 h of hydraulic retention time (HRT) for a wastewater containing 630 mg/L of phenol, corresponding to 1500 mg/L of chemical oxygen demand (COD) and a loading rate of 0.9 g-COD/L/d. The maximum specific methanogenic activity (SMA) of the phenol-degrading sludge was 0.09 g-CH4-COD/g-volatile suspended solids (VSS)/d. Based on 16S rDNA analysis, a total of 21 operational taxonomy units (OTUs) were found in the sludge, of which eight (42.6% of the total population) were related to the sequences in the GenBank with similarity of over 97%, and 13 (79.6%) were affiliated with the known thermophilic species. Additional SMA data and phylogenetic analysis suggest that the degradation pathway of phenol for thermophilic sludge was likely via caproate, instead of benzoate as for the mesophilic sludge.
在55摄氏度、水力停留时间（HRT）为40小时的上流式厌氧污泥床（UASB）反应器中，对于含有630毫克/升苯酚（相当于1500毫克/升化学需氧量（COD））且负荷率为0.9克化学需氧量/升/天的废水，超过99%的苯酚被有效降解。苯酚降解污泥的最大比产甲烷活性（SMA）为0.09克甲烷化学需氧量/克挥发性悬浮固体（VSS）/天。基于16S rDNA分析，在污泥中总共发现了21个操作分类单元（OTU），其中8个（占总数的42.6%）与GenBank中的序列相关，相似度超过97%，13个（占79.6%）隶属于已知的嗜热菌种。额外的SMA数据和系统发育分析表明，嗜热污泥中苯酚的降解途径可能是通过己酸盐，而不是像中温污泥那样通过苯甲酸盐。
