Fang H H P, Liu Y, Ke S Z, Zhang T
Centre for Environmental Engineering Research, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong.
Water Sci Technol. 2004;49(1):95-102.
Treating a synthetic wastewater containing phenol as the sole substrate at 26 degrees C, an upflow anaerobic sludge blanket reactor was able to remove over 98% of phenol up to 1,260 mg/l in wastewater with 12 h of hydraulic retention time, corresponding to 6.0 g-COD/(l x day). Results showed that benzoate was the key intermediate of phenol degradation. Conversion of benzoate to methane was suppressed by the presence of phenol. Based on DNA cloning analysis, the sludge was composed of five groups of microorganisms. Desulfotomaculum and Clostridium were likely responsible for the conversion of phenol to benzoate, which was further degraded by Syntrophus to acetate and H2/CO2. Methanogens lastly converted acetate and H2/CO2 to methane. The role of epsilon-Proteobacteria was, however, unclear.
在26摄氏度下，以上升流式厌氧污泥床反应器处理以苯酚为唯一底物的合成废水，当水力停留时间为12小时时，该反应器能够去除废水中高达1260毫克/升的苯酚，去除率超过98%，相当于6.0克化学需氧量/（升·天）。结果表明，苯甲酸盐是苯酚降解的关键中间产物。苯酚的存在抑制了苯甲酸盐向甲烷的转化。基于DNA克隆分析，该污泥由五组微生物组成。脱硫肠状菌属和梭菌属可能负责将苯酚转化为苯甲酸盐，苯甲酸盐再被互营菌属进一步降解为乙酸盐和氢气/二氧化碳。产甲烷菌最后将乙酸盐和氢气/二氧化碳转化为甲烷。然而，ε-变形菌纲的作用尚不清楚。
