Nishimura Masataka, Shirasawa Hiroshi, Song Wen-Jie
Department of Electronic Engineering, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.
Neurosci Res. 2006 Mar;54(3):230-4. doi: 10.1016/j.neures.2005.12.002. Epub 2006 Jan 9.
We tested the possibility of using a high-power monochromatic InGaN light-emitting diode (LED) as an excitation light source for real-time optical imaging using the voltage-sensitive dye RH-795. Driven with a custom-designed, non-feedback-controlled constant-current circuit, the LED generated stable light with rapid on/off. The LED was comparable with commonly used halogen lamps in exciting RH-795. Acoustically evoked responses in the auditory cortex recorded with the two light sources were highly similar. Our results thus suggest that a high-power LED can be successfully used as an excitation light source for voltage-sensitive dyes, without the need of optical filters and shutters.
我们测试了使用高功率单色氮化铟镓发光二极管（LED）作为激发光源，通过电压敏感染料RH-795进行实时光学成像的可能性。该LED由定制设计的、无反馈控制的恒流电路驱动，能产生具有快速开关特性的稳定光。在激发RH-795方面，该LED与常用的卤素灯相当。用这两种光源记录的听觉皮层中的听觉诱发反应高度相似。因此，我们的结果表明，高功率LED可成功用作电压敏感染料的激发光源，无需光学滤波器和快门。
