Lin Jaung-Gung, Chen Guang-Wei, Li Te-Mao, Chouh Se-Tze, Tan Tzu-Wei, Chung Jing-Gung
School of Chinese Medicine, China Medical University, Taiwan, Republic of China.
J Urol. 2006 Jan;175(1):343-7. doi: 10.1016/S0022-5347(05)00005-4.
We investigated the anticancer effect of AE (1,8-dihydroy-3-[hydroxymethyl]-anthraquione) in the T24 human bladder cancer cell line (Food Industry Research and Development Institute, Hsinchu, Taiwan) by studying apoptosis regulation.
AE, which is purified from aloe vera leaves, has been reported to have antitumor activity. Cell viability, cell cycle and apoptosis were determined by flow cytometric methods. Levels of cyclins, cyclin-dependent kinase 1 and other enzyme were examined by Western blotting methods.
AE inhibited cell viability, and induced G2/M arrest and apoptosis in T24 cells. AE increased the levels of Wee1 and cdc25c, and may have led to inhibition of the levels of cyclin-dependent kinase 1 and cyclin B1, which cause G2/M arrest. AE induced p53 expression and was accompanied by the induction of p21 and caspase-3 activation, which was associated with apoptosis. In addition, AE was associated with a marked increase in Fas/APO1 receptor and Bax expression but it inhibited Bcl-2 expression.
AE induced apoptosis in T24 cells is mediated through the activation of p53, p21, Fas/APO-1, Bax and caspase-3.
我们通过研究凋亡调控，调查了AE（1,8 - 二羟基 - 3 - [羟甲基] - 蒽醌）对T24人膀胱癌细胞系（食品工业发展研究所，台湾新竹）的抗癌作用。
从芦荟叶中纯化得到的AE已被报道具有抗肿瘤活性。通过流式细胞术方法测定细胞活力、细胞周期和凋亡情况。通过蛋白质印迹法检测细胞周期蛋白、细胞周期蛋白依赖性激酶1和其他酶的水平。
AE抑制T24细胞的活力，诱导G2/M期阻滞和凋亡。AE增加了Wee1和cdc25c的水平，可能导致细胞周期蛋白依赖性激酶1和细胞周期蛋白B1水平的抑制，从而引起G2/M期阻滞。AE诱导p53表达，并伴随着p21的诱导和caspase - 3的激活，这与凋亡相关。此外，AE与Fas/APO1受体和Bax表达的显著增加相关，但抑制了Bcl - 2表达。
AE诱导T24细胞凋亡是通过激活p53、p21、Fas/APO - 1、Bax和caspase - 3介导的。
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