DNA甲基转移酶介导的合成辅因子中扩展基团的直接转移。

Direct transfer of extended groups from synthetic cofactors by DNA methyltransferases.

作者信息

Dalhoff Christian, Lukinavicius Grazvydas, Klimasăuskas Saulius, Weinhold Elmar

机构信息

Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, D-52056 Aachen, Germany.

出版信息

Nat Chem Biol. 2006 Jan;2(1):31-2. doi: 10.1038/nchembio754. Epub 2005 Nov 27.

DOI:10.1038/nchembio754

PMID:16408089

Abstract

S-Adenosyl-L-methionine (AdoMet) is the major methyl donor for biological methylation reactions catalyzed by methyltransferases. We report the first chemical synthesis of AdoMet analogs with extended carbon chains replacing the methyl group and their evaluation as cofactors for all three classes of DNA methyltransferases. Extended groups containing a double or triple bond in the beta position to the sulfonium center were transferred onto DNA in a catalytic and sequence-specific manner, demonstrating a high utility of such synthetic cofactors for targeted functionalization of biopolymers.

摘要

S-腺苷-L-甲硫氨酸（AdoMet）是甲基转移酶催化的生物甲基化反应的主要甲基供体。我们报道了首次化学合成具有延长碳链取代甲基的AdoMet类似物，并将其作为所有三类DNA甲基转移酶的辅因子进行评估。在锍中心β位含有双键或三键的延长基团以催化和序列特异性方式转移到DNA上，证明了这种合成辅因子在生物聚合物靶向功能化方面的高度实用性。