面向勇于探索的化学家的测序与光学基因组图谱技术。

Sequencing and Optical Genome Mapping for the Adventurous Chemist.

作者信息

Ruppeka Rupeika Elizabete, D'Huys Laurens, Leen Volker, Hofkens Johan

机构信息

Faculty of Science, Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, Flanders 3001, Belgium.

Perseus Biomics B.V., Industriepark 6 bus 3, Tienen 3300, Belgium.

出版信息

Chem Biomed Imaging. 2024 Oct 25;2(12):784-807. doi: 10.1021/cbmi.4c00060. eCollection 2024 Dec 23.

DOI:10.1021/cbmi.4c00060

PMID:39735829

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11673194/

Abstract

This review provides a comprehensive overview of the chemistries and workflows of the sequencing methods that have been or are currently commercially available, providing a very brief historical introduction to each method. The main optical genome mapping approaches are introduced in the same manner, although only a subset of these are or have ever been commercially available. The review comes with a deck of slides containing all of the figures for ease of access and consultation.

摘要

本综述全面概述了已商业化或目前正在商业化的测序方法的化学原理和工作流程，并对每种方法进行了非常简要的历史介绍。主要的光学基因组图谱绘制方法也以同样的方式介绍，尽管其中只有一部分方法已商业化或曾经商业化。本综述附带一套幻灯片，其中包含所有图表，以便于查阅和参考。