Menéndez V, Villacorta N F, Revilla M A, Gotor V, Bernard P, Fernández H
Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071 Oviedo, Spain.
Plant Cell Rep. 2006 Mar;25(2):85-91. doi: 10.1007/s00299-005-0041-1. Epub 2006 Jan 12.
This work showed for the first time the relationship between the effect of exogenous auxins and gibberellins on apogamy in Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis and its endogenous contents during early apogamic events. The addition of NAA (0.53 and 5.37 microM) or GA(3) 2.8 microM to an MS solid medium significantly increased apogamous sporophyte formation. BA induced brown callus that regenerated sporophytes in a hormone-free medium. The endogenous contents of GA(1), GA(3), GA(4), GA(7), GA(9) and IAA were determined by GC-MS in gametophytes cultured on MS solid medium, before and during early stages of apogamous embryo development. The accumulation of both GA(9) and IAA before embryo development was evident as high levels of GA(4) in the earliest analysed stage of embryo development and high levels of GA(3) in elongating shoots were found. The role of gibberellins on apogamy was also supported by data showing a decrease in the percentage of gametophytes developing embryos because of the addition of flurprimidol to the culture medium.
这项研究首次揭示了外源生长素和赤霉素对相近鳞毛蕨（Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis）无配子生殖的影响与其在早期无配子生殖事件中的内源含量之间的关系。在MS固体培养基中添加萘乙酸（NAA，0.53和5.37微摩尔）或2.8微摩尔的赤霉素（GA₃）可显著增加无配子生殖的孢子体形成。苄氨基嘌呤（BA）诱导产生褐色愈伤组织，该愈伤组织在无激素培养基中再生出孢子体。在无配子生殖胚胎发育之前及早期阶段，利用气相色谱 - 质谱联用（GC-MS）测定了在MS固体培养基上培养的配子体中GA₁、GA₃、GA₄、GA₇、GA₉和吲哚 - 3 - 乙酸（IAA）的内源含量。在胚胎发育之前，GA₉和IAA均有积累，这表现为在胚胎发育最早分析阶段GA₄含量较高，以及在伸长的芽中GA₃含量较高。培养基中添加氟节胺导致发育成胚胎的配子体百分比下降的数据也支持了赤霉素在无配子生殖中的作用。
