Department of Botany, The Maharaja Sayajirao University of Baroda, 390002, Baroda, India.
Plant Cell Rep. 1982 Dec;1(6):261-3. doi: 10.1007/BF00272634.
Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into entire plantlets. An overall multiplication factor of 30-40 was attained within three months.
通过组织培养方法，实现了三叶柄肾蕨属植物的快速无性繁殖。从幼嫩匍匐茎上取下的外植体在添加吲哚丁酸的 B5（ Gamborg 等人，1968）培养基上生长，产生多个叶原基。这些叶原基被分离并培养成完整的幼苗。在三个月内，获得了 30-40 倍的总体繁殖系数。
