Prochukhanova A P, Golovanova A K, Sominina A A, Smirnova T D, Sirotkin A K, Zarubaev V V
Vopr Virusol. 2005 Nov-Dec;50(6):27-30.
The reproduction of the new coronavirus HCoV/SPb/01/03 in the cultured human embryo lung fibroblasts (HELFBs) was electron microscopically studied. The virus was shown to replicate in the cultured HELFBs, by using for this a cell membrane system and causing profound changes in its morphology. After 24 hours of cell infection, there were mature and defective HCoVISPb/01/03 virions were detected in the vacuoles near the peripheral cisterns of the Golgi apparatus. Some of the vacuoles contained folded membranous structures along with virions.
通过电子显微镜研究了新型冠状病毒HCoV/SPb/01/03在培养的人胚肺成纤维细胞（HELFBs）中的复制情况。结果显示该病毒可在培养的HELFBs中复制，为此利用细胞膜系统并使其形态发生了深刻变化。细胞感染24小时后，在高尔基体周边池附近的液泡中检测到成熟和有缺陷的HCoV SPb/01/03病毒粒子。一些液泡中除病毒粒子外还含有折叠的膜结构。
