Dea S, Sawyer N, Alain R, Athanassious R
Centre de Recherche en Virologie, Institut Armand Frappier, Univeresité du Québec, Laval, Canada.
Adv Exp Med Biol. 1995;380:95-8. doi: 10.1007/978-1-4615-1899-0_13.
A Québec reference strain of PRRSV (IAF-KLOP) was successfully propagated in MARC-145 cells, a highly permissive cell clone to PRRSV derived from the MA-104 cell line. Purified extracellular virions appeared as pleomorphic but mostly spherical enveloped particles, 50-72 nm in diameter, with an isometric core about 25-30 nm. By indirect immunofluorescence, detection of viral antigens within the cytoplasm was possible as soon as 6 h.p.i. Nucleocapsids, budding at smooth endoplasmic reticulum (ER), and enveloped viral particles that tended to accumulate in the lumen of ER or Golgi vesicles, were the main features of the viral morphogenesis. The virus apparently was released by exocytosis.
一株魁北克猪繁殖与呼吸综合征病毒（PRRSV）参考毒株（IAF-KLOP）在MARC-145细胞中成功传代，MARC-145细胞是一种对PRRSV高度敏感的细胞克隆，源自MA-104细胞系。纯化的细胞外病毒粒子呈多形性，但大多为球形包膜颗粒，直径50 - 72纳米，有一个约25 - 30纳米的等轴核心。通过间接免疫荧光法，在感染后6小时即可在细胞质中检测到病毒抗原。核衣壳在光滑内质网（ER）处出芽，包膜病毒粒子倾向于积聚在内质网或高尔基体小泡腔中，这是病毒形态发生的主要特征。该病毒显然是通过胞吐作用释放的。
