家羊胃肠道中耐抗生素肠杆菌科细菌的季节性动态变化
Seasonal dynamics of antibiotic-resistant Enterobacteriaceae in the gastrointestinal tract of domestic sheep.
作者信息
Malík R, Ivan J, Javorský P, Pristas P
机构信息
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
出版信息
Folia Microbiol (Praha). 2005;50(4):349-52. doi: 10.1007/BF02931417.
Considerable variation in counts of antibiotic-resistant enterobacteria in the ovine gastrointestinal tract was observed. The occurrence of ruminal and fecal isolates resistant to ampicillin (Ap), kanamycin (Km) and tetracycline (Tc) culminated in summer months, followed by rapid decline in subsequent months. Using PCR the tem1bla (Apr), aphA1 (Kmr) and tetB (Tcr) genes were found to be predominant. Under in vitro conditions all resistance genes were transferable into laboratory Escherichia coli strain with relatively high frequency (10(-3) transconjugants per recipient).
观察到绵羊胃肠道中抗生素抗性肠杆菌的数量存在相当大的差异。对氨苄青霉素（Ap）、卡那霉素（Km）和四环素（Tc）具有抗性的瘤胃和粪便分离株的出现高峰在夏季月份，随后在接下来的几个月中迅速下降。使用聚合酶链反应（PCR）发现，tem1bla（Apr）、aphA1（Kmr）和tetB（Tcr）基因占主导地位。在体外条件下，所有抗性基因都能以相对较高的频率（每受体10^(-3)个转接合子）转移到实验室大肠杆菌菌株中。
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