He Xue-Lin, Li Jian-Ping, Chen Yi-Ping, Zhang Zhi-Gang, Lin Wei-Qin, Chen Jiang-Hua
Nephrology Center, First Hospital, Medical School, Zhejiang University, Hangzhou, Zhejiang Province 310003, China.
Zhong Xi Yi Jie He Xue Bao. 2006 Jan;4(1):43-7. doi: 10.3736/jcim20060112.
To investigate the effects of Tangshenling Mixture (TSLM) and benazepril on rats with diabetic nephropathy (DN) and its mechanism.
Diabetic nephropathy was induced in rats by intraperitoneal injection of streptozotocin. Fifty-eight rats with DN were randomly divided into four groups: untreated group, TSLM-treated group, TSLM plus benazepril-treated group and benazepril-treated group. Another seven normal rats were included in normal control group. Then, rats in each group were accordingly given normal saline, TSLM, TSLM plus benazepril and benazepril orally for six weeks respectively. Blood and urine biochemical indexes, plasma atrial natriuretic factor (ANF), pathomorphology of renal tissue, transforming growth factor beta1 (TGF-beta1) and glucose transporter 1 (GLUT1) mRNAs in renal tissue were observed.
Both TSLM and benazepril could decrease urinary albumin excretion rates, creatinine clearance and ratio of kidney weight to body weight of the rats with DN as well as reduce the pathological damages of the renal tissues. TSLM could reduce the level of plasma ANF and the expression of GLUT1 mRNA, but had no significant effect on the expression of TGF-beta1 mRNA. Benazepril could reduce the expression of TGF-beta1 mRNA, but had no significant effect on plasma ANF and the expression of GLUT1 mRNA.
TSLM can reduce the pathological damages of renal tissues in rats with early-stage DN, and its mechanism may relate to decreasing the level of plasma ANF and the expression of GLUT1 mRNA which is different from that of benazepril. It seems that TSLM has synergetic effect with benazepril.
探讨糖肾灵合剂（TSLM）与贝那普利对糖尿病肾病（DN）大鼠的影响及其作用机制。
通过腹腔注射链脲佐菌素诱导大鼠糖尿病肾病。58只DN大鼠随机分为四组：未治疗组、TSLM治疗组、TSLM加贝那普利治疗组和贝那普利治疗组。另取7只正常大鼠作为正常对照组。然后，分别给予每组大鼠生理盐水、TSLM、TSLM加贝那普利和贝那普利口服，为期六周。观察血、尿生化指标、血浆心钠素（ANF）、肾组织病理形态、肾组织中转化生长因子β1（TGF-β1）和葡萄糖转运蛋白1（GLUT1）mRNA。
TSLM和贝那普利均可降低DN大鼠的尿白蛋白排泄率、肌酐清除率及肾重与体重比值，并减轻肾组织的病理损伤。TSLM可降低血浆ANF水平及GLUT1 mRNA表达，但对TGF-β1 mRNA表达无显著影响。贝那普利可降低TGF-β1 mRNA表达，但对血浆ANF及GLUT1 mRNA表达无显著影响。
TSLM可减轻早期DN大鼠肾组织的病理损伤，其机制可能与降低血浆ANF水平及GLUT1 mRNA表达有关，这与贝那普利不同。TSLM与贝那普利似乎有协同作用。
