Cengiz Salih, Ulukan Ozer, Ates Ismail, Tugcu Harun
Istanbul University, Institute of Legal Medicine and Forensic Sciences, Adli TIP Enstitusu Cerrahpasa, 34301 Istanbul, Turkey.
Forensic Sci Int. 2006 Jan 27;156(2-3):91-4. doi: 10.1016/j.forsciint.2004.12.017.
The aim of this study is to predict how long after time of death a buried body could be analyzed for opiates in soft tissues and to show the accessibility and suitability of bone marrow as a useful toxicological specimen from buried bodies. Morphine solutions were injected in nine albino rabbits. Doses ranged from 0.3 to 1.1 mg/kg with 0.1 mg/kg increments. One hour after the injections, the rabbits were sacrificed. Blood, urine and bone marrow samples were collected for analysis. After the whole bodies were buried, femur bone marrow specimens were collected on the seventh and fourteenth days. CEDIA was used to monitor morphine contents of the collected samples. All experimental cases showed that the increase in the given morphine doses correlated with the increase in blood and bone marrow morphine concentrations. High morphine concentrations were detected in urine samples, but there was no correlation between the urine and blood or urine and bone marrow morphine concentrations. Statistically meaningful increases in bone marrow morphine concentrations were found parallel to increase of blood morphine concentrations. Seventh and fourteenth day postmortem morphine concentrations also followed this correlation. Morphine concentrations in bone marrow at 7 and 14 day postmortem decreased consistently when compared with bone marrow morphine concentrations collected immediately after death. We conclude that in sudden death when other specimens are unavailable due to degradation, bone marrow can be a most useful specimen. Further experimental research in this area is required to validate bone marrow as an alternative tissue.
本研究的目的是预测一具埋葬尸体的软组织中鸦片类药物分析可在死亡时间后多久进行，并表明骨髓作为埋葬尸体有用的毒理学标本的可获取性和适用性。向9只白化兔注射吗啡溶液。剂量范围为0.3至1.1毫克/千克，以0.1毫克/千克递增。注射1小时后，处死兔子。采集血液、尿液和骨髓样本进行分析。将整个尸体埋葬后，在第7天和第14天采集股骨骨髓标本。采用CEDIA监测采集样本中的吗啡含量。所有实验病例均显示，给予的吗啡剂量增加与血液和骨髓吗啡浓度增加相关。在尿液样本中检测到高吗啡浓度，但尿液与血液或尿液与骨髓吗啡浓度之间无相关性。发现骨髓吗啡浓度有统计学意义的增加与血液吗啡浓度的增加平行。死后第7天和第14天的吗啡浓度也遵循这种相关性。与死后立即采集的骨髓吗啡浓度相比，死后第7天和第14天骨髓中的吗啡浓度持续下降。我们得出结论，在猝死时，当由于降解而无法获得其他标本时，骨髓可能是最有用的标本。需要在该领域进行进一步的实验研究以验证骨髓作为替代组织的可行性。
