Hédouin V, Bourel B, Bécart A, Tournel G, Deveaux M, Goff M L, Gosset D
Institut de Médecine Légale et de Médecine Sociale, Faculté de Médecine, Lille, France.
J Forensic Sci. 2001 Jan;46(1):12-4.
Two species of blow flies (Diptera: Calliphoridae) were reared on tissues from rabbits administered different dosages of morphine. These species, Protophormia terraenovae and Calliphora vicina are among the first wave of insects colonizing a dead body. Two series of 3 rabbits were given dosages of 10, 20, and 40 mg/h of morphine over a 3 h period via ear artery perfusion. A morphine blood level plateau was attained after 1 h of perfusion. Two other rabbits were used as controls. Samples of tissues collected from rabbits using a coelioscopic technique were determined to have morphine concentrations similar to those encountered in human overdoses and were correlated with dosages of morphine administered. All samples from control rabbits were negative for morphine. Larvae and puparia of both species were regularly collected from each rabbit for toxicological analysis. Concentrations of morphine in larvae reared on rabbit carcasses containing morphine were significantly lower than concentrations found in the tissues. There was a decrease in concentration in morphine observed in transition from feeding 3rd instar larva to puparium. A correlation between larval concentration and tissue concentration was found only in feeding 3rd instar larvae.
在给兔子注射不同剂量吗啡后，用其组织饲养了两种丽蝇（双翅目：丽蝇科）。这两种丽蝇，即新陆原伏蝇和红头丽蝇，是最早在尸体上定殖的昆虫之一。通过耳动脉灌注，对两组各3只兔子在3小时内分别给予10、20和40毫克/小时的吗啡剂量。灌注1小时后达到吗啡血药浓度平台期。另外两只兔子用作对照。使用腹腔镜技术从兔子身上采集的组织样本经测定，其吗啡浓度与人类过量用药时的浓度相似，且与所给予的吗啡剂量相关。所有对照兔子的样本吗啡检测均为阴性。定期从每只兔子收集这两种丽蝇的幼虫和蛹进行毒理学分析。在含有吗啡的兔子尸体上饲养的幼虫体内，吗啡浓度显著低于在组织中发现的浓度。从取食的三龄幼虫到蛹，观察到吗啡浓度有所下降。仅在取食的三龄幼虫中发现幼虫浓度与组织浓度之间存在相关性。
