基质金属蛋白酶-9和-2在小儿分泌性中耳炎中的表达
Expression of matrix metalloproteinase-9 and -2 in pediatric chronic otitis media with effusion.
作者信息
Jang Chul-Ho, Shin Sung-Heui, Cho Hyong-Ho, Moon Seok-Jin, Cho Yong-Bum
机构信息
Department of Otolaryngology, Chonnam National University Medical School, Hak-dong 8, Gwangju, South Korea.
出版信息
Int J Pediatr Otorhinolaryngol. 2006 Jul;70(7):1155-8. doi: 10.1016/j.ijporl.2005.11.013. Epub 2006 Jan 18.
OBJECTIVE
To evaluate the role of MMP-9 and MMP-2 in pediatric patients with middle ear effusion (MEE) and determine the pathogenesis of otitis media with effusion (OME) and allergy.
METHODS
The MEE samples were collected from 25 patients with allergy and 20 patients without allergy as a control. The levels of MMP-9 and MMP-2 were measured using a commercially available enzyme-linked immunosorbent assay (ELISA). The activity of MMP-9 and MMP-2 were measured by gelatin-zymography. The Mann-Whitney U-test was used for comparisons between the allergy positive and control groups.
RESULTS
The level of MMP-9 was significantly elevated in MEE from the allergy positive group compared to controls. The amount of MMP-9 activity significantly increased in the allergy positive group compared to controls.
CONCLUSION
MMP-9 and MMP-2 are mediators of inflammation in the OME; in addition, MMP-9 may play a significant role in the pathophysiology of OME with allergy.
目的
评估基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶-2（MMP-2）在小儿中耳积液（MEE）患者中的作用，并确定分泌性中耳炎（OME）和过敏的发病机制。
方法
收集25例过敏患者和20例无过敏患者的MEE样本作为对照。使用市售的酶联免疫吸附测定（ELISA）法测量MMP-9和MMP-2的水平。通过明胶酶谱法测量MMP-9和MMP-2的活性。采用曼-惠特尼U检验对过敏阳性组和对照组进行比较。
结果
与对照组相比，过敏阳性组MEE中MMP-9水平显著升高。与对照组相比，过敏阳性组MMP-9活性显著增加。
结论
MMP-9和MMP-2是OME炎症的介质；此外，MMP-9可能在伴有过敏的OME病理生理过程中起重要作用。
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