Harakeh Steve, Yassine Hadi, El-Fadel Mutasem
Department of Biology, American University of Beirut, P.O. Box 11-0236, Beirut, Lebanon.
Environ Pollut. 2006 Sep;143(2):269-77. doi: 10.1016/j.envpol.2005.11.027. Epub 2006 Jan 18.
This study is the first to be conducted in Lebanon on the isolation and molecular characterization and the antimicrobial resistance profile of environmental pathogenic bacterial strains. Fifty-seven samples of seawater, sediment, crab, and fresh water were collected during the spring and summer seasons of 2003. The isolation of Escherichia coli and Salmonella using appropriate selective media revealed that 94.7% of the tested samples were contaminated with one or both of the tested bacteria. The polymerase chain reaction (PCR) was then used to identify the species of both bacteria using various sets of primers. Many pathogenic E. coli isolates were detected by PCR out of which two were identified as O157:H7 E. coli. Similarly, the species of many of the Salmonella isolates was molecularly identified. The confirmed isolates of Salmonella and E. coli were then tested using the disk diffusion method for their susceptibility to four different antimicrobials revealing high rates of antimicrobial resistance.
本研究是在黎巴嫩首次针对环境致病性细菌菌株的分离、分子特征及抗菌药物耐药性进行的研究。2003年春夏季期间采集了57份海水、沉积物、螃蟹及淡水样本。使用适当的选择性培养基分离大肠杆菌和沙门氏菌，结果显示94.7%的受试样本被一种或两种受试细菌污染。随后采用聚合酶链反应（PCR），使用多组引物鉴定这两种细菌的种类。通过PCR检测到许多致病性大肠杆菌分离株，其中两株被鉴定为O157:H7大肠杆菌。同样，许多沙门氏菌分离株的种类也通过分子方法得以鉴定。然后，采用纸片扩散法对确诊的沙门氏菌和大肠杆菌分离株进行四种不同抗菌药物的敏感性测试，结果显示抗菌药物耐药率很高。
