Carvalho Fátima C T, Sousa Oscarina V, Carvalho Edirsana M R, Hofer Ernesto, Vieira Regine H S F
Laboratório de Microbiologia Ambiental e do Pescado, Instituto de Ciências do Mar (LABOMAR), Universidade Federal do Ceará, Avenida Abolicão 3207, CEP 60165-081, Fortaleza, CE, Brazil.
Laboratório de Zoonoses Bacterianas, Instituto Oswaldo Cruz (FIOCRUZ)-Pavilhão Rocha Lima, Avenida Brasil 4365, CEP 21040-360, Manguinhos-Rio de Janeiro, RJ, Brazil.
J Pathog. 2013;2013:685193. doi: 10.1155/2013/685193. Epub 2013 Dec 22.
This study investigated the presence and antibiotic resistance of Salmonella spp. in a shrimp farming environment in Northeast Region of Brazil. Samples of water and sediments from two farms rearing freshwater-acclimated Litopenaeus vannamei were examined for the presence of Salmonella. Afterwards, Salmonella isolates were serotyped, the antimicrobial resistance was determined by a disk diffusion method, and the plasmid curing was performed for resistant isolates. A total of 30 (16.12%) of the 186 isolates were confirmed to be Salmonella spp., belonging to five serovars: S. serovar Saintpaul, S. serovar Infantis, S. serovar Panama, S. serovar Madelia, and S. serovar Braenderup, along with 2 subspecies: S. enterica serovar houtenae and S. enterica serovar enterica. About twenty-three percent of the isolates were resistant to at least one antibiotic, and twenty percent were resistant to at least two antibiotics. Three strains isolated from water samples (pond and inlet canal) exhibited multiresistance to ampicillin, tetracycline, oxytetracycline, and nitrofurantoin. One of them had a plasmid with genes conferring resistance to nitrofurantoin and ampicillin. The incidence of bacteria pathogenic to humans in a shrimp farming environment, as well as their drug-resistance pattern revealed in this study, emphasizes the need for a more rigorous attention to this area.
本研究调查了巴西东北地区对虾养殖环境中沙门氏菌属的存在情况及其抗生素耐药性。对两个养殖淡水驯化凡纳滨对虾的养殖场的水和沉积物样本进行了沙门氏菌检测。之后，对沙门氏菌分离株进行血清分型，采用纸片扩散法测定其抗菌耐药性，并对耐药分离株进行质粒消除。在186株分离株中，共有30株（16.12%）被确认为沙门氏菌属，属于5个血清型：圣保罗沙门氏菌、婴儿沙门氏菌、巴拿马沙门氏菌、马德利亚沙门氏菌和布伦德鲁普沙门氏菌，以及2个亚种：肠炎沙门氏菌豪滕亚种和肠炎沙门氏菌肠炎亚种。约23%的分离株对至少一种抗生素耐药，20%的分离株对至少两种抗生素耐药。从水样（池塘和进水渠）中分离出的3株菌株对氨苄西林、四环素、土霉素和呋喃妥因表现出多重耐药性。其中一株含有携带对呋喃妥因和氨苄西林耐药基因的质粒。本研究揭示的对虾养殖环境中对人类致病细菌的发生率及其耐药模式，强调了对此领域需要给予更严格关注。
