Lu Y Q, Zhang M, Meng B, Lu S S, Wei Y M, Lu K H
Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, China.
Anim Reprod Sci. 2006 Sep;95(1-2):158-64. doi: 10.1016/j.anireprosci.2005.11.005. Epub 2006 Jan 18.
The aim of this study was to identify the difference in DNA content characterizing the X- and Y-chromosome bearing sperm of buffalo. Sperm from six Murrah buffaloes and six Nili-Ravi buffaloes were collected and stained with Hoechst 33342 followed by flow cytometry analysis of the DNA content. Two symmetrical, separate but overlapping peaks presumed to be X- and Y-chromosome bearing sperm were detected. The difference in fluorescence intensity, which related to the DNA content, between the X- and Y-sperm was 3.59+/-0.11% for Murrah buffalo and 3.55+/-0.14% for Nili-Ravi buffalo, respectively. Significant differences were observed among males within each breed, but there were no differences between the averages of the two breeds. The results indicate that flow cytometric sorting of X- and Y-sperm of buffalo is feasible.
本研究的目的是确定表征水牛携带X和Y染色体精子的DNA含量差异。采集了6头摩拉水牛和6头尼里-拉菲水牛的精子，用Hoechst 33342染色，随后对DNA含量进行流式细胞术分析。检测到两个对称、分离但重叠的峰，推测分别为携带X和Y染色体的精子。摩拉水牛X精子和Y精子之间与DNA含量相关的荧光强度差异分别为3.59±0.11%，尼里-拉菲水牛为3.55±0.14%。在每个品种的雄性个体之间观察到显著差异，但两个品种的平均值之间没有差异。结果表明，对水牛的X精子和Y精子进行流式细胞术分选是可行的。
