Stark Yvonne, Suck Kirstin, Kasper Cornelia, Wieland Martin, van Griensven Martijn, Scheper Thomas
Institute for Technical Chemistry, University of Hannover, Callinstr. 3, 30167 Hannover, Germany.
Exp Toxicol Pathol. 2006 Mar;57(4):305-11. doi: 10.1016/j.etp.2005.10.005. Epub 2006 Jan 18.
Articular cartilage shows little capacity for self-repair once it has been damaged. The aim of this study was to investigate different collagen matrices regarding their applicability for cartilage tissue engineering. The matrices consist of collagen I and small amounts of elastine, were crosslinked with carbodiimide or glucose. Primary chondrocytes were seeded onto these different collagen matrices and cultured with or without differentiation medium. The viability of the cells was monitored via MTT test. The arrangement of the cells onto the scaffold was investigated by histological staining. Furthermore, extracellular matrix synthesis was studied by immunohistological staining, especially the expression of the typical chondrogenic marker collagen II. Moreover gene expression for collagen type II was analysed by RT-PCR. The chondrocytes showed high viability on all matrices used. The results for the histological staining revealed a three-dimensional arrangement of the chondrocytes in the collagen matrices. Moreover, the matrices also supported chondrogenic differentiation. On the matrix MATRIDERM 2 mm the synthesis of collagen II was stimulated without adding any differentiation supplements to the cell culture medium, as observed by immunohistological staining and by gene expression analysis of collagen II.
关节软骨一旦受损，自我修复能力很差。本研究的目的是研究不同的胶原基质在软骨组织工程中的适用性。这些基质由I型胶原和少量弹性蛋白组成，用碳二亚胺或葡萄糖交联。将原代软骨细胞接种到这些不同的胶原基质上，并在有或没有分化培养基的情况下进行培养。通过MTT试验监测细胞活力。通过组织学染色研究细胞在支架上的排列。此外，通过免疫组织学染色研究细胞外基质的合成，特别是典型软骨生成标志物II型胶原的表达。此外，通过RT-PCR分析II型胶原的基因表达。软骨细胞在所有使用的基质上都表现出高活力。组织学染色结果显示软骨细胞在胶原基质中呈三维排列。此外，这些基质也支持软骨生成分化。通过免疫组织学染色和II型胶原基因表达分析观察到，在不向细胞培养基中添加任何分化补充剂的情况下，在MATRIDERM 2 mm基质上II型胶原的合成受到刺激。
