Akhtar M J, Khan S U, Khan M A
Research and Development Laboratories, Welcome Pakistan Ltd., D/43, S.I.T.E., Karachi, Pakistan.
Pak J Pharm Sci. 1993 Jan;6(1):29-34.
The knowledge of UV absorption characteristics of paracetatmol, pseudoephedrine HCl and triprolidine HCl has been used to determine these compounds by HPLC method in pharmaceutical preparations. The method involves reverse phase chromatography with isocratic mode of elusion. Rather selecting a compromised wavelength for all the three components, "wavelength switching" programme has been executed to give reasonable UV responses because of the widely different UV characteristics of these components. This method performs well in term of the precision and accuracy as indicated by linear regression analysis. The RSD of the method is 1.12 to 2.87% and the recovery is 103.10-95.56%.
对乙酰氨基酚、盐酸伪麻黄碱和盐酸曲普利啶的紫外线吸收特性已用于通过高效液相色谱法测定药物制剂中的这些化合物。该方法采用等度洗脱的反相色谱法。由于这些组分的紫外线特性差异很大，因此未为所有三种组分选择折衷波长，而是执行了“波长切换”程序以给出合理的紫外线响应。线性回归分析表明，该方法在精密度和准确度方面表现良好。该方法的相对标准偏差为1.12%至2.87%，回收率为103.10 - 95.56%。
