Akhtar M J, Khan S, Hafiz M
Research and Development Laboratories, Wellcome Pakistan Limited, Karachi.
J Pharm Biomed Anal. 1994 Mar;12(3):379-82. doi: 10.1016/0731-7085(94)90014-0.
The different UV absorption characteristics of paracetamol, pseudoephedrine hydrochloride and triprolidine hydrochloride have been used to facilitate their determination in pharmaceutical preparations by HPLC. The method developed involves isocratic, reversed-phase chromatography. A 'wavelength switching' programme is preferred to the use of a compromise wavelength for all three compounds. This gives reasonable UV responses despite widely different UV characteristics and concentrations of drugs. The limits of quantitation are found to be within 200-700 micrograms ml-1 for paracetamol, 24-84 micrograms ml-1 for pseudoephedrine HCl and 1.0-4.0 micrograms ml-1 for triprolidine HCl. The method performs well in terms of precision and accuracy as indicated by linear regression analysis.
