Monagas María, Hernández-Ledesma Blanca, Gómez-Cordovés Carmen, Bartolomé Begoña
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
J Agric Food Chem. 2006 Jan 25;54(2):319-27. doi: 10.1021/jf051807j.
This paper reports an attempt to functionally and chemically characterize commercial ingredients from Vitis vinifera L. grape skins, grape pomace, and leaves, which are used in the formulation of dietary antioxidant supplements. The antioxidant capacity of these ingredients was assessed for the first time by the oxygen radical absorbance capacity (ORAC) methodology. Ingredients from grape skins and pomace (n = 17) showed ORAC values from 1.38 to 21.4 mumol Trolox equivalents/mg whereas ingredients from leaves (n = 4) showed ORAC values from 1.52 to 2.55 mumol Trolox equivalents/mg. The high-performance liquid chromatography-diode array detection/electrospray ionization-mass sprectrometry analysis of anthocyanins and flavonols revealed the authenticity of the ingredients as derived from V. vinifera L. and confirmed large differences in their phenolic content and distribution. A progressive decline in both antioxidant capacity and total anthocyanin content of a grape skin ingredient (43 and 40% decrease, respectively) was observed over a 60 day storage period (45 degrees C and 75% relative humidity), demonstrating its poor stability under these conditions.
本文报道了一项针对用于膳食抗氧化剂补充剂配方的葡萄（Vitis vinifera L.）皮、葡萄渣和叶子中的商业成分进行功能和化学特性分析的尝试。首次采用氧自由基吸收能力（ORAC）方法评估了这些成分的抗氧化能力。葡萄皮和葡萄渣中的成分（n = 17）的ORAC值为1.38至21.4微摩尔Trolox当量/毫克，而叶子中的成分（n = 4）的ORAC值为1.52至2.55微摩尔Trolox当量/毫克。对花青素和黄酮醇进行的高效液相色谱 - 二极管阵列检测/电喷雾电离 - 质谱分析揭示了这些成分源自葡萄（V. vinifera L.）的真实性，并证实了它们在酚类含量和分布上存在很大差异。在60天的储存期（45摄氏度和75%相对湿度）内，观察到一种葡萄皮成分的抗氧化能力和总花青素含量均逐渐下降（分别下降43%和40%），表明其在这些条件下稳定性较差。
