Groch K M, Clifton K H
University of Wisconsin Clinical Cancer Center, Department of Human Oncology, Madison 53792.
Acta Endocrinol (Copenh). 1992 Jun;126(6):515-23. doi: 10.1530/acta.0.1260515.
A fraction of enzymatically monodispersed rat thyrocytes from untreated animals clonally proliferate into thyroid follicular units following transplantation into the subcutaneous fat pads of syngeneic recipients. During the induction of experimental goiters in rats either with 3-amino-1,2,4-triazole/iodine sufficient diet or KClO4/Remington low iodine diet, the clonogenic fractions of cells from aminotriazole goiters decreased to 1.9 x 10(-4) and KClO4 goiters to 9.8 x 10(-5) as compared to 5.8 x 10(-3) for cells from age-matched controls during the growth phase of goitrogenesis. With continued aminotriazole treatment after thyroid hyperplasia had ceased, the clonogenic fraction increased to 2.0 x 10(-3) while continued KClO4 treatment had little further effect. The changes in the clonogenic fraction induced by both regimens were reversed during involution; goitrogenic rechallenge of involuted glands led to changes in the clonogenic fraction similar to that noted during the initial challenge. The clonogenic fractions of cells from aminotriazole goiters were greater than that of cells from KClO4 goiters at all time points examined despite similar TSH levels in situ. We conclude that the rat thyroid contains a hierarchy of cells with different proliferative capacities and that the clonogenic thyrocytes possess many of the attributes of a stem-cell.
将未经处理动物的酶促单分散大鼠甲状腺细胞移植到同基因受体的皮下脂肪垫后，其中一部分会克隆性增殖形成甲状腺滤泡单位。在用3-氨基-1,2,4-三唑/碘充足饮食或高氯酸钾/雷明顿低碘饮食诱导大鼠实验性甲状腺肿的过程中，与甲状腺肿发生生长阶段年龄匹配对照的细胞相比，氨基三唑甲状腺肿细胞的克隆形成率降至1.9×10⁻⁴，高氯酸钾甲状腺肿细胞的克隆形成率降至9.8×10⁻⁵。在甲状腺增生停止后继续用氨基三唑处理，克隆形成率增加到2.0×10⁻³，而继续用高氯酸钾处理则几乎没有进一步影响。两种方案诱导的克隆形成率变化在退化过程中逆转；对退化腺体进行致甲状腺肿再激发导致克隆形成率变化与初次激发时相似。尽管原位促甲状腺激素水平相似，但在所有检测时间点，氨基三唑甲状腺肿细胞的克隆形成率均高于高氯酸钾甲状腺肿细胞。我们得出结论，大鼠甲状腺含有具有不同增殖能力的细胞层级，且克隆性甲状腺细胞具有许多干细胞的特征。
