Groch K M, Clifton K H
University of Wisconsin Clinical Cancer Center, Department of Human Oncology, Madison 53792.
Acta Endocrinol (Copenh). 1992 Jan;126(1):85-96. doi: 10.1530/acta.0.1260085.
In the rat, chronic TSH stimulation leads to self-limited thyroid hyperplasia, goitrogenesis, and TSH-responsive thyroid tumors. The current studies were aimed at clarifying the mechanism by which hormone-responsive, proliferating follicular cells arise in quiescent plateau phase rat goiters. Enzymatically monodispersed rat thyrocytes from early plateau phase and involuting goiters were analyzed for the capacity to form thyroid follicular units after transplantation into syngeneic recipients. Goiters induced with two different regimens contained substantial numbers of cells capable of proliferating into functioning thyroid follicular tissue after transplantation. The clonogenic fractions of cells from goiters induced by 3-amino-1,2,4-triazole or KClO4/Remington low iodine diet were significantly lower than that of cells from control glands. Furthermore, the clonogenic fraction of cells from the KClO4 goiters was also found to be significantly less than that of cells from aminotriazole goiters despite similar TSH levels in the cell donors. The hormone responsiveness of the clonogenic goiter cells and the histological appearance and functional capacity of the thyroid tissue which arose after grafting were indistinguishable from controls. We conclude that the capacity to clonally proliferate into follicular units is a specific trait which characterizes a unique subset of follicular cells and speculate that the hormone-responsive tumors which typically appear in the chronically stimulated rat thyroid originate from cells within this subset.
在大鼠中，慢性促甲状腺激素（TSH）刺激会导致自限性甲状腺增生、甲状腺肿形成以及对TSH有反应的甲状腺肿瘤。当前的研究旨在阐明在静止期平台期大鼠甲状腺肿中出现激素反应性、增殖性滤泡细胞的机制。将来自早期平台期和消退期甲状腺肿的大鼠甲状腺细胞酶解分散后，分析其移植到同基因受体中形成甲状腺滤泡单位的能力。用两种不同方案诱导的甲状腺肿含有大量细胞，这些细胞在移植后能够增殖形成有功能的甲状腺滤泡组织。由3-氨基-1,2,4-三唑或高氯酸钾/雷明顿低碘饮食诱导的甲状腺肿细胞的克隆形成率明显低于对照腺体的细胞。此外，尽管细胞供体中的TSH水平相似，但高氯酸钾甲状腺肿细胞的克隆形成率也明显低于氨基三唑甲状腺肿的细胞。克隆性甲状腺肿细胞的激素反应性以及移植后出现的甲状腺组织的组织学外观和功能能力与对照无明显差异。我们得出结论，克隆增殖形成滤泡单位的能力是一种特定特征，它表征了滤泡细胞的一个独特亚群，并推测在慢性刺激的大鼠甲状腺中通常出现的激素反应性肿瘤起源于该亚群中的细胞。
