Adil H, Sheikh D, Ahmed I, Sheikh M R, Ali G
Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi.
Pak J Pharm Sci. 1995 Jul;8(2):1-10.
The efficacy of zinc sulphate and boric acid ophthalmic preparations, containing benzalkonium chloride as preservative have been investigated against Gram-negative Escherichia coli and Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. The viability of bacteria was checked after 7 days though they were completely suppressed after 24 hours of inoculation. Zinc sulphate increased the antimicrobial activity of benzalkonium chloride, whereas the boric acid or its combination with zinc sulphate reduced the same. The Pseudomonas aeruginosa was found to be viable against zinc sulphate, boric acid or its combination at a concentration of 0.01% to 0.005% of the preservative. Escherichia coil and Staphylococcus aureus also exhibited resistance but to a lesser extent than Pseudomonas aeruginosa. This viability may be dangerous in case of multidose ophthalmic preparations.
对含有苯扎氯铵作为防腐剂的硫酸锌和硼酸眼科制剂针对革兰氏阴性大肠杆菌、铜绿假单胞菌以及革兰氏阳性金黄色葡萄球菌的功效进行了研究。尽管接种24小时后细菌被完全抑制，但在7天后检查了细菌的生存能力。硫酸锌增强了苯扎氯铵的抗菌活性，而硼酸或其与硫酸锌的组合则降低了这种活性。发现在防腐剂浓度为0.01%至0.005%时，铜绿假单胞菌对硫酸锌、硼酸或其组合具有生存能力。大肠杆菌和金黄色葡萄球菌也表现出耐药性，但程度低于铜绿假单胞菌。对于多剂量眼科制剂而言，这种生存能力可能是危险的。
