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利用紫外共振拉曼光谱法表征细菌生长及抗生素的影响。

Characterization of bacterial growth and the influence of antibiotics by means of UV resonance Raman spectroscopy.

作者信息

Neugebauer U, Schmid U, Baumann K, Holzgrabe U, Ziebuhr W, Kozitskaya S, Kiefer W, Schmitt M, Popp J

机构信息

Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, Jena 07743, Germany.

出版信息

Biopolymers. 2006 Jul;82(4):306-11. doi: 10.1002/bip.20447.

Abstract

In this work we monitor the bacterial growth of a Bacillus pumilus batch culture by means of UV resonance Raman spectroscopy. Excitation with a wavelength of 244 nm especially enhances the Raman scattering of the aromatic amino acids and the nucleic acid bases and therefore is a good method to track the metabolic changes that occur during bacterial growth. Furthermore, a drug from the fluoroquinolone group is added to the bacterial suspension at the beginning of the exponential growth phase. With the help of chemometrical methods such as hierarchical cluster analysis (HCA) and principal component analysis (PCA) it is possible to visualize the small changes that occur in the UV resonance Raman spectra due to the interaction of the drug with its biological targets DNA and the enzyme gyrase within the bacterial cell.

摘要

在这项工作中,我们通过紫外共振拉曼光谱法监测短小芽孢杆菌分批培养物的细菌生长情况。波长为244 nm的激发光特别增强了芳香族氨基酸和核酸碱基的拉曼散射,因此是追踪细菌生长过程中发生的代谢变化的良好方法。此外,在指数生长阶段开始时,将一种氟喹诺酮类药物添加到细菌悬浮液中。借助化学计量学方法,如层次聚类分析(HCA)和主成分分析(PCA),可以可视化由于药物与其生物靶点DNA以及细菌细胞内的酶促旋酶相互作用而在紫外共振拉曼光谱中发生的微小变化。

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