Kaneko Takamasa, Makimura Koichi, Onozaki Masanobu, Ueda Kumiko, Yamada Yohko, Nishiyama Yayoi, Yamaguchi Hideyo
Kanto Chemical Co., Inc. Marusan BLDG. 11-5, Nihonbashi Honcho, 3-Chome, Chuo-ku, Tokyo 103-0023, Japan.
Med Mycol. 2005 Dec;43(8):699-704. doi: 10.1080/13693780500130564.
A comparison of several media, i.e., potato dextrose agar with olive oil (Oil-PDA), modified Dixon agar (mDIX) and variations of Leeming and Notman agar (LNA) for the isolation and growth of Malassezia and Candida species was examined. Since LNA supported the highest growth of Malassezia species its key components, i.e., ox bile, glycerol monostearate, glycerol and Tween 60, were added to CHROMagar Candida. All 7 species of Malassezia grew well on this modified medium (LN-CHROM) after incubation for 4 days at 30 degrees C and development was equal to that observed on LNA. Colonies on LN-CHROM were smooth and from pink to dark purple in color. Furthermore, the use of LN-CHROM did not alter the colony characteristics of Candida species as compared to that found on CHROMagar Candida. The results of the present investigation indicate that the use of LN-CHROM would make possible the simultaneous isolation and identification of Malassezia and Candida species.
研究了几种培养基，即添加橄榄油的马铃薯葡萄糖琼脂（油-PDA）、改良迪克森琼脂（mDIX）以及用于马拉色菌和念珠菌分离与生长的利明和诺特曼琼脂（LNA）的变体。由于LNA支持马拉色菌的最高生长，因此将其关键成分，即牛胆汁、单硬脂酸甘油酯、甘油和吐温60添加到显色念珠菌琼脂中。在30℃孵育4天后，所有7种马拉色菌在这种改良培养基（LN-显色琼脂）上生长良好，生长情况与在LNA上观察到的相当。LN-显色琼脂上的菌落光滑，颜色从粉红色到深紫色。此外，与显色念珠菌琼脂上的情况相比，使用LN-显色琼脂不会改变念珠菌的菌落特征。本研究结果表明，使用LN-显色琼脂将使同时分离和鉴定马拉色菌和念珠菌成为可能。
