: A Selective Culture Medium for Species.

species are fastidious and slow-growing yeasts in which isolation from polymicrobial samples is hampered by fast-growing microorganisms. selective culture media are needed. Although cycloheximide is often used, some fungi, including the chief human commensal , are resistant to this compound. This study aimed to test whether the macrolide rapamycin could be used in combination with cycloheximide to develop a -selective culture medium. Rapamycin susceptibility testing was performed via microdilution assays in modified Dixon against two and five spp. The MIC was the lowest concentration that reduced growth by a minimum of 90%. Rapamycin ± cycloheximide 500 mg/L was also added to FastFung solid, and yeast suspensions were inoculated and incubated for 72 h. Rapamycin MICs for spp. ranged from 0.5 to 2 mg/L, except for , for which the MIC was >32 mg/L. stains were rapamycin-resistant. Rapamycin and cycloheximide supplementation of the FastFung medium effectively inhibited the growth of non- yeast, including cycloheximide-resistant and . Based on our findings, this "" medium should be further evaluated on polymicrobial samples for isolation and culture.