Pinheiro Thiátira S D B, Johansson Loana A P, Pizzolatti Moacir G, Biavatti Maique W
Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Curso de Farmácia, CCS, University of Vale do Itajaí (Univali), Rua: Uruguai 458, Caixa Postal 360, Itajai, Santa Catarina (SC) 88302-202, Brazil.
J Pharm Biomed Anal. 2006 May 3;41(2):431-6. doi: 10.1016/j.jpba.2005.12.010. Epub 2006 Jan 19.
The leaves of the pantropical genus Bauhinia (Fabaceae) are popularly known as cow's-paw or cow's hoof due to their unique characteristic bilobed aspect. The species Bauhinia forficata (Brazilian Orchid-tree) is widely used in folk medicine as an antidiabetic. This article deals with the quantitative analysis of kaempferitrin from B. forficata medicinal extract (aqueous and hydro alcoholic) using the LC method, and the comparison of kaempferitrin content in leaves collected from two different regions in the south Brazil. The total flavonoid content assessed by LC was also compared with the classical spectrophotometric determination. Kaempferitrin was found in different amounts, in samples from two geographical areas (Telêmaco Borba/PR and Itajaí/SC), for aqueous (368.68 and 77.91 microg/mL) and hydro alcoholic extracts (1952.59 and 211.61 microg/mL), respectively. The method was subjected to recovery assay, to determine its accuracy. A marked difference in total flavonoid concentration was observed in relation to kaempferitrin content: 2759.95 and 2188.20 microg/mL for the fluidextract and 863.35 and 856.77 microg/mL for the aqueous extract (Telêmaco Borba/PR and Itajaí/SC). The spectrophotometric assay overestimated the total flavonoid content (3620 microg/mL) in relation to the LC assay.
泛热带的羊蹄甲属（豆科）植物的叶子因其独特的二裂形态而被通俗地称为牛蹄叶。羊蹄甲（巴西兰花树）在民间医学中被广泛用作抗糖尿病药物。本文采用液相色谱法对羊蹄甲药用提取物（水提取物和水醇提取物）中的山柰酚苷进行定量分析，并比较巴西南部两个不同地区采集的叶子中山柰酚苷的含量。还将通过液相色谱法评估的总黄酮含量与经典的分光光度法测定结果进行比较。在来自两个地理区域（特莱马科博尔巴/巴拉那州和伊塔雅伊/圣卡塔琳娜州）的样品中，分别在水提取物（368.68和77.91微克/毫升）和水醇提取物（1952.59和211.61微克/毫升）中发现了不同含量的山柰酚苷。该方法进行了回收率测定，以确定其准确性。观察到总黄酮浓度与山柰酚苷含量存在显著差异：流浸膏为2759.95和2188.20微克/毫升，水提取物为863.35和856.77微克/毫升（特莱马科博尔巴/巴拉那州和伊塔雅伊/圣卡塔琳娜州）。与液相色谱法测定相比，分光光度法高估了总黄酮含量（3620微克/毫升）。
