Tomaso Herbert, Bartling Carsten, Al Dahouk Sascha, Hagen Ralf M, Scholz Holger C, Beyer Wolfgang, Neubauer Heinrich
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany.
Syst Appl Microbiol. 2006 Jan;29(1):24-8. doi: 10.1016/j.syapm.2005.05.008. Epub 2005 Jul 1.
Anthrax Blood Agar (ABA) and Cereus Ident Agar (CEI) were evaluated as selective growth media for the isolation of Bacillus anthracis using 92 B. anthracis and 132 other Bacillus strains from 30 species. The positive predictive values for the identification of B. anthracis on ABA, CEI, and the combination of both were 72%, 71%, and 90%, respectively. Thus, less than 10% of all species were misidentified using both nutrient media. Species which might be misidentified as B. anthracis were B. cereus, B. mycoides, and B. thuringiensis. Particularly, 30% of B. weihenstephanensis strains were misidentified as B. anthracis.
使用来自30个物种的92株炭疽芽孢杆菌和132株其他芽孢杆菌菌株,对炭疽血琼脂(ABA)和蜡样芽孢杆菌鉴定琼脂(CEI)作为分离炭疽芽孢杆菌的选择性生长培养基进行了评估。在ABA、CEI以及两者组合培养基上鉴定炭疽芽孢杆菌的阳性预测值分别为72%、71%和90%。因此,使用这两种营养培养基时,所有物种中被误鉴定的比例不到10%。可能被误鉴定为炭疽芽孢杆菌的物种有蜡样芽孢杆菌、蕈状芽孢杆菌和苏云金芽孢杆菌。特别是,30%的魏氏芽孢杆菌菌株被误鉴定为炭疽芽孢杆菌。