Harmon S M
J Assoc Off Anal Chem. 1982 Sep;65(5):1134-9.
A collaborative study was conducted of a new method for differentiating members of the Bacillus cereus group. Using the new method, each of 14 collaborators identified 8 Bacillus cultures, which represented 3 biotypes of the B. cereus group. Each culture was tested for motility, hemolytic activity on trypticase-soy-sheep blood agar, and rhizoid growth on nutrient agar; carbol-fuchsin stain was used to determine the presence of protein toxin crystals. Cultures were identified as B. cereus (biotype 1), B. cereus var. mycoides (biotype 2), or B. thuringiensis (biotype 3). All collaborators correctly identified the unknown cultures and classified them correctly as to biotype. There were no statistically significant differences in the identification rates among the different laboratories. Additional tests by one participant on 5 strains of Bacillus anthracis showed that the new method is also adequate for differentiating B. anthracis from typically reacting strains of B. cereus. The method has been adopted interim official first action.
开展了一项关于区分蜡样芽孢杆菌群成员新方法的合作研究。使用该新方法，14位合作者每人鉴定了8株芽孢杆菌培养物，这些培养物代表蜡样芽孢杆菌群的3种生物型。对每种培养物进行了运动性测试、在胰蛋白酶大豆-绵羊血琼脂上的溶血活性测试以及在营养琼脂上的根状生长测试；使用石炭酸品红染色来确定蛋白质毒素晶体的存在。培养物被鉴定为蜡样芽孢杆菌（生物型1）、蕈状芽孢杆菌（生物型2）或苏云金芽孢杆菌（生物型3）。所有合作者都正确鉴定了未知培养物，并将它们正确分类到生物型。不同实验室之间的鉴定率没有统计学上的显著差异。一名参与者对5株炭疽芽孢杆菌菌株进行的额外测试表明，该新方法也足以将炭疽芽孢杆菌与典型反应的蜡样芽孢杆菌菌株区分开来。该方法已被采用为暂行官方首次行动。
