通过多重聚合酶链反应检测空肠弯曲菌中的cdtA、cdtB和cdtC基因。
Detection of cdtA, cdtB, and cdtC genes in Campylobacter jejuni by multiplex PCR.
作者信息
Martínez Irati, Mateo Estibaliz, Churruca Estibaliz, Girbau Cecilia, Alonso Rodrigo, Fernández-Astorga Aurora
机构信息
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Paseo de la Universidad, 7 1, E-01006 Vitoria-Gasteiz, Spain.
出版信息
Int J Med Microbiol. 2006 Feb;296(1):45-8. doi: 10.1016/j.ijmm.2005.08.003. Epub 2005 Nov 18.
A multiplex PCR was developed for simultaneous detection of the cytolethal distending toxin (cdt) genes of Campylobacter jejuni. Three primer pairs targeting each one of the cdtA, cdtB and cdtC genes were designed and combined in the same PCR reaction. The assay was evaluated with 100 C. jejuni strains recovered from humans and animals and it was found to be rapid and specific. Two isolates presented several deletions affecting both cdtA and cdtB genes. High prevalence (98%) of the three cdt genes was found among isolates of different geographic origins.
开发了一种多重聚合酶链反应（PCR）用于同时检测空肠弯曲菌的细胞致死膨胀毒素（cdt）基因。设计了针对cdtA、cdtB和cdtC基因的三对引物，并将它们组合在同一个PCR反应中。用从人和动物中分离出的100株空肠弯曲菌菌株对该检测方法进行了评估，发现其快速且具有特异性。有两株分离株出现了影响cdtA和cdtB基因的多处缺失。在不同地理来源的分离株中发现这三种cdt基因的高流行率（98%）。
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