CBQF/Biotechnology School, Portuguese Catholic University, Porto, Portugal.
Foodborne Pathog Dis. 2010 Jan;7(1):103-6. doi: 10.1089/fpd.2009.0326.
From a collection of 242 food isolates of Campylobacter spp. 72 were identified as Campylobacter jejuni and 170 as Campylobacter coli and of 112 isolates from clinical sources, 92 were identified as C. jejuni and 20 as C. coli. The results for food isolates were in contrast to other reported identifications. Twenty C. coli strains from clinical cases of Campylobacter that occurred in Portugal and 170 isolated from poultry products were examined for the presence of cytolethal distending toxin (cdt) by multiplex polymerase chain reaction assay. CdtA, cdtB, and cdtC were present in 100% of the clinical strains. With respect to the food isolates, the cdtA and cdtC genes were detected in 99.4% of the strains and cdtB in 98.8%. Small point mutations or major deletions within the primer binding sites are possible explanations for these observations but need to be further investigated.
从 242 株弯曲菌属食品分离株中，72 株鉴定为空肠弯曲菌，170 株鉴定为大肠弯曲菌；从临床来源的 112 株分离株中，92 株鉴定为空肠弯曲菌，20 株鉴定为大肠弯曲菌。食品分离株的结果与其他报道的鉴定结果相反。通过多重聚合酶链反应检测，对来自葡萄牙发生的弯曲菌临床病例的 20 株大肠弯曲菌菌株和 170 株从家禽产品中分离的菌株进行细胞致死扩张毒素 (cdt) 的存在情况。临床菌株中 100%存在 cdtA、cdtB 和 cdtC。对于食品分离株，99.4%的菌株中检测到 cdtA 和 cdtC 基因，98.8%的菌株中检测到 cdtB 基因。引物结合位点的小点突变或主要缺失可能是造成这些观察结果的原因，但需要进一步调查。
