Whittle Gabrielle, Hamburger Nathan, Shoemaker Nadja B, Salyers Abigail A
Department of Microbiology, 601 S. Goodwin Ave., University of Illinois, Urbana, IL 61801, USA.
J Bacteriol. 2006 Feb;188(3):1169-74. doi: 10.1128/JB.188.3.1169-1174.2006.
CTnERL, a Bacteroides conjugative transposon, transferred DNA by an Hfr-type mechanism during conjugation when it was excision deficient due to an insertion in the integrase gene. Rescue of the conjugative transposon sequences required the recipient to be RecA proficient and to contain an integrated CTnERL. The transfer efficiency was only 10- to 30-fold lower than the normal element transfer efficiency, and the direction of transfer from the oriT gene showed that the integrase end was transferred first and that the transfer genes were transferred last.
CTnERL是一种拟杆菌属接合转座子,当它因整合酶基因中的插入而切除缺陷时,在接合过程中通过Hfr型机制转移DNA。接合转座子序列的拯救要求受体RecA功能正常且含有整合的CTnERL。转移效率仅比正常元件转移效率低10至30倍,并且从oriT基因的转移方向表明整合酶末端首先被转移,转移基因最后被转移。