Shivaji S, Jagannadham M V
Centre for Cellular and Molecular Biology, Hyderabad, India.
Biochim Biophys Acta. 1992 Jul 8;1108(1):99-109. doi: 10.1016/0005-2736(92)90119-7.
The interaction of progesterone, 17-alpha-hydroxyprogesterone, testosterone and estradiol with membrane vesicles prepared from phosphatidylserine (PS), from the total lipids of human and hamster spermatozoa, from the lipids of hamster spermatozoal plasma and acrosomal membrane and with the native membranes of hamster spermatozoa have been investigated by 90 degrees light scattering and fluorescence spectroscopy. The results indicate that progesterone decreases the fluidity of membranes, aggregates membrane vesicles, induces fusion of membrane vesicles and also renders them permeable to hydrophilic molecules like carboxyfluorescein. But, testosterone and estradiol at the same concentration had very little effect on membrane fluidity, membrane aggregation, fusion and leakage. The above membrane perturbing activities of the steroids is discussed in light of the recent findings that progesterone induces acrosome reaction in human and hamster spermatozoa [11,18].
通过90度光散射和荧光光谱法，研究了孕酮、17-α-羟基孕酮、睾酮和雌二醇与由磷脂酰丝氨酸（PS）制备的膜囊泡、与人及仓鼠精子总脂质制备的膜囊泡、与仓鼠精子质膜和顶体膜脂质制备的膜囊泡以及与仓鼠精子天然膜之间的相互作用。结果表明，孕酮降低了膜的流动性，使膜囊泡聚集，诱导膜囊泡融合，还使它们对羧基荧光素等亲水分子具有通透性。但是，相同浓度的睾酮和雌二醇对膜流动性、膜聚集、融合和渗漏的影响很小。根据孕酮诱导人和仓鼠精子顶体反应的最新研究结果[11,18]，讨论了类固醇上述的膜扰动活性。
