Osman R A, Andria M L, Jones A D, Meizel S
Department of Human Anatomy, University of California, Davis 95616.
Biochem Biophys Res Commun. 1989 Apr 28;160(2):828-33. doi: 10.1016/0006-291x(89)92508-4.
By a combination of organic precipitation and high pressure liquid chromatography, human sperm acrosome reaction inducing activity has been purified from the fluid aspirated from preovulatory human ovarian follicles and identified as 4-pregnen-3,20-dione (progesterone) and 4-pregnen-17 alpha-ol-3,20-dione (17 alpha-hydroxyprogesterone). It is argued that progesterone is present at the site of fertilization of placental mammals in concentrations sufficient for activity, and hence provides a mechanism of inducing the acrosome reaction, an exocytotic event, in vivo.
通过有机沉淀和高压液相色谱相结合的方法，从排卵前人类卵巢卵泡抽吸液中纯化出了诱导人类精子顶体反应的活性物质，并鉴定为4-孕烯-3,20-二酮（孕酮）和4-孕烯-17α-醇-3,20-二酮（17α-羟孕酮）。有人认为，胎盘哺乳动物受精部位存在的孕酮浓度足以产生活性，因此提供了一种在体内诱导顶体反应（一种胞吐事件）的机制。
