Igarashi S, Kano Y, Nishita T, Amasaki H, Asari M
Toxicology Research Laboratories, Chugai Pharmaceutical Co., Ltd., Nagano, Japan.
J Vet Med Sci. 1992 Jun;54(3):535-9. doi: 10.1292/jvms.54.535.
The present paper described the immunohistochemical distributions of carbonic anhydrase (CA) isozymes. CA-I, CA-II and CA-III, in the epithelium lining the rat gastrointestinal tract, with rabbit antibodies to equine CA-I, CA-II and CA-III. Prior to the immunohistochemical examinations, the crossreactivities of these antibodies to the rat-antigens were confirmed in this study. In the stomach, surface epithelial cells and parietal cells of the glandular region showed an immunoreactivity only to CA-II. In the large intestine, each immunoreactivity to CA isozyme (CA-I, CA-II and CA-III) was localized in the upper portion of intestinal glands, and decreased toward the distal digestive tract, but absent in the small intestine. The present histological findings suggested that the CA isozymes might play a role in the ion-transportation during the water absorption in the rat large intestine.
本文描述了碳酸酐酶（CA）同工酶CA-I、CA-II和CA-III在大鼠胃肠道上皮中的免疫组织化学分布，使用了针对马CA-I、CA-II和CA-III的兔抗体。在进行免疫组织化学检查之前，本研究证实了这些抗体与大鼠抗原的交叉反应性。在胃中，腺区的表面上皮细胞和壁细胞仅对CA-II呈免疫反应性。在大肠中，对CA同工酶（CA-I、CA-II和CA-III）的每种免疫反应性都定位于肠腺的上部，并向消化道远端降低，但在小肠中不存在。目前的组织学研究结果表明，CA同工酶可能在大鼠大肠水吸收过程中的离子转运中发挥作用。
