Parkkila S, Parkkila A K, Juvonen T, Rajaniemi H
Department of Anatomy, University of Oulu, Finland.
Gut. 1994 May;35(5):646-50. doi: 10.1136/gut.35.5.646.
The distribution of carbonic anhydrase isoenzymes I, II, and VI was studied in the human alimentary tract using specific antibodies to human isoenzymes in conjunction with the immunoperoxidase technique to elucidate the physiological role and possible functional interplay of carbonic anhydrases (CAs) in alimentary canal functions. From the isoenzymes studied, CA II was found to be the most widely distributed in the various epithelia throughout the alimentary canal. In addition to the acinar cells of the parotid and submandibular glands and the duodenal Brunner's glands, it was present in the mucosal epithelium of the oesophagus, stomach, duodenum, and colon. The epithelial cells of the hepatic bile ducts, gall bladder, and pancreatic ducts also contained CA II in abundance. In contrast, CA VI was present only in the serous acinar and ductal cells of the parotid and submandibular glands, and CA I in the mucosal epithelium of the colon and the A cells of the pancreatic Langerhans's islets. These results suggest that CA II as a widely distributed isoenzyme in the epithelia of the alimentary canal and CA VI as secreted into saliva, may form a mutually complementary system protecting oesophageal, gastric, and intestinal mucosa from acidity.
利用针对人同工酶的特异性抗体结合免疫过氧化物酶技术，研究了碳酸酐酶同工酶I、II和VI在人体消化道中的分布，以阐明碳酸酐酶（CAs）在消化道功能中的生理作用及可能的功能相互作用。在所研究的同工酶中，发现CA II在整个消化道的各种上皮细胞中分布最广。除了腮腺和颌下腺的腺泡细胞以及十二指肠的布伦纳腺外，它还存在于食管、胃、十二指肠和结肠的黏膜上皮中。肝内胆管、胆囊和胰管的上皮细胞中也大量含有CA II。相比之下，CA VI仅存在于腮腺和颌下腺的浆液性腺泡和导管细胞中，而CA I存在于结肠的黏膜上皮和胰腺胰岛的A细胞中。这些结果表明，作为在消化道上皮中广泛分布的同工酶的CA II以及分泌到唾液中的CA VI，可能形成一个相互补充的系统，保护食管、胃和肠黏膜免受酸性物质的侵害。
