Spicer S S, Ge Z H, Tashian R E, Hazen-Martin D J, Schulte B A
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425.
Am J Anat. 1990 Jan;187(1):55-64. doi: 10.1002/aja.1001870107.
Carbonic anhydrase (CA) III was demonstrated immunocytochemically in epithelium in some regions of salivary gland ducts, colon, bronchi, and male genital tract and in adipocytes, in addition to skeletal muscle and liver where the isozyme was previously localized. Basal cells beneath the submandibular gland's excretory ducts in guinea pig stained for CA III. Carbonic anhydrase III occurred alone in some and with CA II in other sites but was often absent from CA-II-containing types of cells. This was exemplified by CA III's abundance in CA-II-positive proximal colon and its sparsity in the CA-II-rich distal colon of the mouse. Striated ducts in guinea pig, but not mouse salivary glands, stained darker for CA and appeared accordingly to function more actively in ion transport compared with excretory ducts. Carbonic anhydrase content varied among genera in liver and pancreas and between mouse species and strains in salivary glands and kidney. Newly observed murine sites of CA II activity included Auerbach's plexus and a population of leukocytes infiltrating the lamina propria in small intestine, and several types of cells in the male genital tract. In immunoblot tests, antisera to CA III showed no cross reactivity with antisera to CA II, but those to CA II disclosed weak cross reactivity with CA III.
碳酸酐酶（CA）III除了在先前已定位该同工酶的骨骼肌和肝脏中被免疫细胞化学证实存在外，还在唾液腺导管、结肠、支气管和男性生殖道的某些区域的上皮细胞以及脂肪细胞中被发现。豚鼠下颌下腺排泄导管下方的基底细胞对CA III呈阳性染色。碳酸酐酶III在一些部位单独存在，在其他部位与CA II共存，但在含有CA II的细胞类型中通常不存在。这在小鼠中表现为CA III在CA II阳性的近端结肠中丰富，而在富含CA II的远端结肠中稀少。豚鼠的纹状管对CA染色比小鼠唾液腺的纹状管更深，因此与排泄导管相比，其在离子转运中似乎功能更活跃。碳酸酐酶含量在肝脏和胰腺的不同属之间以及唾液腺和肾脏的不同小鼠物种和品系之间有所不同。新观察到的CA II在小鼠中的活性部位包括奥尔巴赫神经丛、浸润小肠固有层的一群白细胞以及男性生殖道中的几种细胞类型。在免疫印迹试验中，CA III抗血清与CA II抗血清无交叉反应，但CA II抗血清与CA III有弱交叉反应。
