Diaz A O, Escalante A H, García A M, Goldemberg A L
Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250 3rd piso, 7600 Mar del Plata, Argentina.
Anat Histol Embryol. 2006 Feb;35(1):42-6. doi: 10.1111/j.1439-0264.2005.00654.x.
The histology of the pharyngeal cavity and oesophagus of the freshwater 'silverside'Odontesthes bonariensis (Cuvier and Valenciennes) and the characteristics of their mucous cells were investigated. The histological characterization of its digestive wall revealed that the mucosa is thrown with longitudinal folds. The epithelium covering the folds was stratified with abundant mucous cells and gustative corpuscles, which are lacking in the oesophagus. The muscularis mucosa was absent. The submucosa presented the compactum stratum. The muscularis was organized in longitudinal and circular layers of muscular striated fibres. The serosa with a flat epithelium was located only in the oesophagus. Using histochemical procedures including methods for localization and characterization of glycoproteins (GPs), no differences were detected between the mucous cells contents of the pharyngeal cavity and those of the oesophagus. The mucous cells showed a weak periodic acid-Schiff (PAS) positive reaction in their content. The reactions for the differential analysis of sialic acids from GPs are feeble for periodic acid-Schiff at low temperature and low pH (PAS) and KOH/PAS and strong for periodic acid/borohydride/KOH/PAS (PA/Bh/KOH/PAS) and KOH/PA*/Bh/PAS revealing the scarce presence of C7 or C9 substituted and non-substituted sialic acids and the abundance of C7, C8 substituted sialic acids, O-acyl sugars and neutral sugars respectively. The results suggest that the pharyngeal cavity with the gustative corpuscles would induce the gastric secretion whereas the oesophagus is mainly involved in the transport of the food bolus to the stomach with the aid of abundant secretion of mucus. GPs secreted on the surface of the mucous cells, likely related to environmental conditions, would be involved in the lubrication, protection against abrasion and inhibition of microorganism proliferation.
对淡水“银汉鱼”布氏南美拟沙丁鱼（居维叶和瓦朗谢纳）的咽腔和食管组织学及其黏液细胞特征进行了研究。其消化壁的组织学特征显示，黏膜有纵向褶皱。覆盖褶皱的上皮为复层，有丰富的黏液细胞和味小体，食管中则没有味小体。黏膜肌层不存在。黏膜下层有致密层。肌层由纵向和环形的横纹肌纤维层组成。仅在食管中有扁平上皮的浆膜。使用包括糖蛋白（GPs）定位和表征方法在内的组织化学程序，未检测到咽腔和食管黏液细胞内容物之间的差异。黏液细胞内容物对过碘酸希夫（PAS）呈弱阳性反应。对于GPs中唾液酸的差异分析反应，在低温和低pH值下过碘酸希夫（PAS）和KOH/PAS反应较弱，而过碘酸/硼氢化物/KOH/PAS（PA/Bh/KOH/PAS）和KOH/PA*/Bh/PAS反应较强，分别揭示了C7或C9取代和未取代唾液酸的稀缺存在以及C7、C8取代唾液酸、O-酰基糖和中性糖的丰富存在。结果表明，有味小体的咽腔会诱导胃液分泌，而食管主要借助大量黏液分泌将食团输送到胃。黏液细胞表面分泌的GPs可能与环境条件有关，参与润滑、防止磨损和抑制微生物增殖。
