Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250 3°piso, 7600 Mar del Plata, Argentina.
J Fish Biol. 2010 Nov;77(7):1665-73. doi: 10.1111/j.1095-8649.2010.02803.x. Epub 2010 Oct 15.
The histochemistry of glycoproteins (GP) in the mucous cells of the gills of the silverside Odontesthes bonariensis was identified with: (1) oxidizable vicinal diols; (2) sialic acid and some of their chain variants, carbon 7 ((7) C), carbon 8 ((8) C) or carbon 9 ((9) C); (3) sialic acid residues without O-acyl substitution and with O-acyl substitution at (7) C, (8) C or (9) C; (4) carboxyl groups and (5) sulphate groups. A battery of seven biotinylated lectins allowed GPs sugar residues to be distinguished. Mucous cells showed the presence of neutral, sulphated and sialylated GPs. Dolichos biflorus agglutinin (DBA) and Glycine max agglutinin (SBA) showed strong positive staining; Arachis hypogaea agglutinin (PNA), Ricinus communis agglutinin-I (RCA-I) and Triticum vulgaris agglutinin (WGA) showed moderate staining, while Ulex europaeus agglutinin-I (UEA-I) was completely negative.
对银汉鱼鳃黏液细胞中的糖蛋白（GP）的组织化学进行了鉴定：（1）可氧化的顺式二醇；（2）唾液酸及其一些链变体，碳 7（(7) C）、碳 8（(8) C）或碳 9（(9) C）；（3）无 O-酰基取代和在（7）C、（8）C 或（9）C 处具有 O-酰基取代的唾液酸残基；（4）羧基和（5）硫酸根。一套七种生物素化的凝集素允许区分 GP 糖残基。黏液细胞显示存在中性、硫酸化和唾液酸化的 GP。双花扁豆凝集素（DBA）和 Glycine max 凝集素（SBA）显示强阳性染色；花生凝集素（PNA）、蓖麻凝集素-I（RCA-I）和普通小麦凝集素（WGA）显示中度染色，而荆豆凝集素-I（UEA-I）则完全阴性。
