Kozhura Vyacheslav Leontjevich, Basarab Dmitriy Alexeevich, Timkina Marina Innokentievna, Golubev Arkadiy Mikhailivich, Reshetnyak Vasiliy Ivanovich, Moroz Viktor Vasiljevich
Laboratory of Experimental Therapy, Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow 107031, Russia.
World J Gastroenterol. 2005 Dec 7;11(45):7084-90. doi: 10.3748/wjg.v11.i45.7084.
To investigate the anti-ischemic properties of perfluorochemical emulsion "perftoran" in mesenteric region.
Experiments were conducted on 146 nonlinear white male rats weighing 200-350 g. Partial critical intestinal ischemia was induced by thorough atraumatic strangulation of 5-6 cm jejunal loop with its mesentery for 90 min. Global critical intestinal ischemia was made by atraumatic occlusion of the cranial mesenteric artery (CMA) for 90 min also. Perftoran (PF, 0.8-1.0 mL per 100 g) in experimental groups or 0.9% sodium chloride in control groups was injected at 75 min of ischemic period. Mean systemic arterial blood pressure (BP(M)) registration, intravital microscopy and morphological examination of ischemic intestine and its mesentery were performed in both groups.
During 90 min of reperfusion, BP(M) progressively decreased to 27.3+/-7.4% after PF administration vs 38.6+/-8.0% in the control group of rats with partial intestinal ischemia (NS) and to 50.3+/-6.9% vs 53.1+/-5.8% in rats after global ischemia (NS). During the reperfusion period, full restoration of microcirculation was never registered; parts with restored blood flow had leukocyte and erythrocyte stasis and intra-vascular clotting, a typical "non-reflow" phenomenon. The reduction of mesenteric 50-400 mum feeding artery diameter was significantly less in the PF group than in the control group (24+/-5.5% vs 45.2+/-3.6%, P<0.05) 5 min after partial intestinal ischemia. This decrease progressed but differences between groups minimized at the 90(th) min of reperfusion (41.5+/-4.2% and 50.3+/-2.8%, respectively). In reperfusion of rat's intestine, a significant mucosal alteration was registered. Villous height decreased 2.5-3 times and the quantity of crypts decreased more than twice. In the group of rats administered PF, intestinal mucosal layer was protected from irreversible post-ischemic derangement during reperfusion. Saved cryptal epithelial cells were the source of regeneration of the epithelium, which began to cover renewing intestinal villi after 24 h of blood flow restoration. View of morphological alterations was more heterogeneous in CMA groups.
Systemic administration of perftoran promotes earlier and more complete structural regeneration during reperfusion in rats after partial and global critical intestinal ischemia.
研究全氟化合物乳剂“全氟托兰”在肠系膜区域的抗缺血特性。
对146只体重200 - 350克的非线性雄性白色大鼠进行实验。通过对5 - 6厘米空肠袢及其肠系膜进行彻底无创伤性绞窄90分钟诱导部分临界性肠缺血。通过无创伤性阻断肠系膜上动脉（CMA）90分钟造成全身性临界性肠缺血。在缺血期75分钟时，给实验组注射全氟托兰（PF，每100克0.8 - 1.0毫升），对照组注射0.9%氯化钠。两组均进行平均体循环动脉血压（BP(M)）记录、活体显微镜检查以及缺血肠段及其肠系膜的形态学检查。
在90分钟再灌注期间，部分肠缺血大鼠给予PF后，BP(M)逐渐下降至27.3±7.4%，而对照组为38.6±8.0%（无显著差异）；全身性缺血大鼠给予PF后BP(M)降至50.3±6.9%，对照组为53.1±5.8%（无显著差异）。在再灌注期间，从未记录到微循环的完全恢复；血流恢复的部位存在白细胞和红细胞淤滞以及血管内凝血，这是典型的“无复流”现象。部分肠缺血5分钟后，PF组肠系膜50 - 400微米供血动脉直径的减小明显小于对照组（24±5.5%对45.2±3.6%，P<0.05）。这种减小持续进展，但在再灌注90分钟时两组间差异最小（分别为41.5±4.2%和50.3±2.8%）。在大鼠肠再灌注过程中，记录到明显的黏膜改变。绒毛高度降低2.5 - 3倍，隐窝数量减少超过两倍。在给予PF的大鼠组中，肠黏膜层在再灌注期间免受不可逆的缺血后紊乱影响。保存下来的隐窝上皮细胞是上皮再生的来源，在血流恢复24小时后开始覆盖更新的肠绒毛。CMA组的形态学改变视图更不均匀。
全身性给予全氟托兰可促进部分和全身性临界性肠缺血大鼠再灌注期间更早且更完全的结构再生。
