Hungerhuber Edwin, Zausinger Stefan, Westermaier Thomas, Plesnila Nikolaus, Schmid-Elsaesser Robert
Institute for Surgical Research, Klinikum Grosshadern, University of Munich, Germany.
J Neurosci Methods. 2006 Jun 30;154(1-2):109-15. doi: 10.1016/j.jneumeth.2005.12.004. Epub 2006 Jan 24.
Laser Doppler fluxmetry (LDF) and electrocorticography (ECG) are techniques used to indicate successful occlusion of the middle cerebral artery (MCAO) in the intraluminal filament model of ischemic stroke. However, each method has several advantages and drawbacks. This article describes a simple technique to simultaneously and continuously monitor LDF and ECG over both cerebral hemispheres. We investigated the potential of this method to improve the reliability of the filament model. Thirty male Sprague-Dawley rats were subjected to transient MCAO under three different experimental conditions (n=10 each group): MCAO in [A] normothermic animals, in [B] animals treated with hypothermia and in [C] animals receiving barbiturate for induction of burst suppression. Cortical blood flow was continuously recorded bilaterally by LDF and the electrocorticogram was continuously recorded over both hemispheres. The results show that monitoring of cortical electrophysiological activity by ECG allows detection of subarachnoid hemorrhage (SAH) during normal electrophysiological status and provides continuous control of barbiturate induced burst suppression as well as information about postischemic electrophysiological recovery. ECG did not detect MCAO, premature reperfusion, or SAH during burst suppression induced by barbiturates. In contrast, MCAO, SAH and premature reperfusion were rapidly indicated by LDF. Our findings suggest that simultaneous bilateral LDF and ECG during MCAO are of complementary value, in particular if barbiturates are investigated.
激光多普勒血流测定法（LDF）和皮层脑电图（ECG）是用于在缺血性中风的管腔内丝线模型中指示大脑中动脉（MCAO）成功闭塞的技术。然而，每种方法都有若干优点和缺点。本文描述了一种在两个大脑半球同时连续监测LDF和ECG的简单技术。我们研究了该方法提高丝线模型可靠性的潜力。将30只雄性Sprague-Dawley大鼠在三种不同实验条件下（每组n = 10）进行短暂性MCAO：[A]正常体温动物的MCAO，[B]低温治疗动物的MCAO，以及[C]接受巴比妥酸盐诱导爆发抑制的动物的MCAO。通过LDF双侧连续记录皮层血流，并在两个半球连续记录皮层脑电图。结果表明，通过ECG监测皮层电生理活动可在正常电生理状态下检测蛛网膜下腔出血（SAH），并提供对巴比妥酸盐诱导的爆发抑制的持续控制以及关于缺血后电生理恢复的信息。在巴比妥酸盐诱导的爆发抑制期间，ECG未检测到MCAO、过早再灌注或SAH。相比之下，LDF可快速指示MCAO、SAH和过早再灌注。我们的研究结果表明，在MCAO期间同时进行双侧LDF和ECG具有互补价值，特别是在研究巴比妥酸盐时。
