Department of Clinical Chemistry and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
BMC Neurosci. 2018 Apr 19;19(1):24. doi: 10.1186/s12868-018-0425-0.
Stroke is among the leading causes of death and disability. Although intense research efforts have provided promising treatment options in animals, most clinical trials in humans have failed and the therapeutic options are few. Several factors have been suggested to explain this translational difficulty, particularly concerning methodology and study design. Consistent infarcts and low mortality might be desirable in some, but not all, studies. Here, we aimed to investigate whether the use of laser Doppler flowmetry (LDF) and the occlusion time (60 vs. 45 min) affected outcome variability and mortality in a rat stroke model. Eighty ovariectomized female Wistar rats were subjected to ischemic stroke using intraluminal filament middle cerebral artery occlusion with or without LDF and with occlusion times of 45 or 60 min. Outcome was evaluated by triphenyl tetrazolium chloride staining of brain slices to measure infarct size and a modified sticky tape test.
Neither LDF nor occlusion times of 45 versus 60 min significantly affected mortality, outcome variability or outcome severity.
Due to the unexpectedly high mortality and variability the statistical power was very low and thus the results were inconclusive.
中风是导致死亡和残疾的主要原因之一。尽管大量的研究努力为动物提供了有前途的治疗选择，但大多数人类临床试验都失败了，治疗选择很少。有几个因素被认为可以解释这种转化困难，特别是在方法学和研究设计方面。在某些研究中，一致的梗死和低死亡率可能是可取的，但并非所有研究都如此。在这里，我们旨在研究激光多普勒流量测量（LDF）和闭塞时间（60 分钟与 45 分钟）的使用是否会影响大鼠中风模型的结果变异性和死亡率。80 只去卵巢雌性 Wistar 大鼠通过管腔内纤维蛋白大脑中动脉闭塞，使用或不使用 LDF，并采用 45 分钟或 60 分钟的闭塞时间。通过脑切片的三苯基四唑氯化物染色来测量梗死面积和改良的粘性胶带测试来评估结果。
LDF 或 45 分钟与 60 分钟的闭塞时间均未显著影响死亡率、结果变异性或结果严重程度。
由于出乎意料的高死亡率和变异性，统计效力非常低，因此结果不确定。
