Schmid-Elsaesser R, Zausinger S, Hungerhuber E, Baethmann A, Reulen H J
Department of Neurosurgery, Klinikum Grosshadern, Ludwig-Maximilians-University, Munich, Germany
Stroke. 1998 Oct;29(10):2162-70. doi: 10.1161/01.str.29.10.2162.
The intraluminal thread model for middle cerebral artery occlusion (MCAO) has gained increasing acceptance. Numerous modifications have been reported in the literature, indicating that the technique has not been standardized. The present study was performed to evaluate and optimize the reliability of this model.
One hundred Sprague-Dawley rats were subjected to MCAO by 2 different intraluminal filaments. Cortical blood flow was continuously monitored over both hemispheres by laser-Doppler flowmetry (LDF). In part I (3-0 filament), we evaluated the incidence of adequate MCAO, subarachnoid hemorrhage (SAH), intraluminal thrombus formation, and the effects of heparinization. In part II (silicone-coated 4-0 filament), we also determined the influence of insufficient MCAO on morphological and functional outcome and the incidence of postischemic hyperthermia.
In part I, SAH occurred in 30% and premature reperfusion in 24%. All animals with a decrease in contralateral flow had suffered SAH. Thrombus formation was not observed in any group. In part II, SAH occurred in 8% and premature reperfusion in 26%. There was no difference in outcome between rats with primary MCAO and rats with filament correction. Animals with uncorrected premature reperfusion had significantly smaller infarct volumes and fewer neurological deficits.
SAH and insufficient MCAO may be more common in the intraluminal thread model than previously reported. Inadvertent premature reperfusion contributes to the interanimal variability associated with this model. The incidence of valid experiments increases with the use of a silicone-coated 4-0 filament. Continuous bilateral LDF is indispensable to monitor adequate MCAO and is highly sensitive to recognize SAH.
大脑中动脉闭塞（MCAO）的管腔内丝线模型已越来越被广泛接受。文献中报道了许多该模型的改良方法，这表明该技术尚未标准化。本研究旨在评估并优化此模型的可靠性。
100只Sprague-Dawley大鼠通过2种不同的管腔内丝线进行MCAO。通过激光多普勒血流仪（LDF）连续监测双侧大脑半球的皮质血流。在第一部分（3-0丝线）中，我们评估了充分MCAO、蛛网膜下腔出血（SAH）、管腔内血栓形成的发生率以及肝素化的影响。在第二部分（硅胶涂层4-0丝线）中，我们还确定了不充分MCAO对形态学和功能结局的影响以及缺血后体温过高的发生率。
在第一部分中，SAH发生率为30%，过早再灌注发生率为24%。所有对侧血流减少的动物均发生了SAH。任何组均未观察到血栓形成。在第二部分中，SAH发生率为8%，过早再灌注发生率为26%。原发性MCAO大鼠和丝线校正大鼠的结局无差异。未校正过早再灌注的动物梗死体积明显较小，神经功能缺损较少。
SAH和不充分的MCAO在管腔内丝线模型中可能比先前报道的更常见。意外的过早再灌注导致了该模型相关的动物间变异性。使用硅胶涂层4-0丝线可提高有效实验的发生率。连续双侧LDF对于监测充分的MCAO是必不可少的，并且对识别SAH高度敏感。
