Laurencikiene Jurga, Källman Annika M, Fong Nova, Bentley David L, Ohman Marie
Department of Molecular Biology and Functional Genomics, Stockholm University, 106 91 Stockholm, Sweden.
EMBO Rep. 2006 Mar;7(3):303-7. doi: 10.1038/sj.embor.7400621. Epub 2006 Jan 27.
The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (pol II) is essential for several co-transcriptional pre-messenger RNA processing events, including capping, 3'-end processing and splicing. We investigated the role of the CTD of RNA pol II in the coordination of A to I editing and splicing of the ADAR2 (ADAR: adenosine deaminases that act on RNA) pre-mRNA. The auto-editing of Adar2 intron 4 by the ADAR2 adenosine deaminase is tightly coupled to splicing, as the modification of the dinucleotide AA to AI creates a new 3' splice site. Unlike other introns, the CTD is not required for efficient splicing of intron 4 at either the normal 3' splice site or the alternative site created by editing. However, the CTD is required for efficient co-transcriptional auto-editing of ADAR2 intron 4. Our results implicate the CTD in site-selective RNA editing by ADAR2 and in coordination of editing with alternative splicing.
RNA聚合酶II（pol II）大亚基的羧基末端结构域（CTD）对于几种共转录前体信使RNA加工事件至关重要，包括加帽、3'末端加工和剪接。我们研究了RNA pol II的CTD在ADAR2（ADAR：作用于RNA的腺苷脱氨酶）前体mRNA的A到I编辑与剪接协调中的作用。ADAR2腺苷脱氨酶对Adar2内含子4的自我编辑与剪接紧密偶联，因为二核苷酸AA修饰为AI会产生一个新的3'剪接位点。与其他内含子不同，无论是在正常的3'剪接位点还是由编辑产生的替代位点，CTD对于内含子4的有效剪接都不是必需的。然而，CTD对于ADAR2内含子4的有效共转录自我编辑是必需的。我们的结果表明CTD参与了ADAR2的位点选择性RNA编辑以及编辑与可变剪接的协调。
