实时荧光定量PCR法对日本学龄前儿童牙菌斑中变形链球菌的定量检测

Quantitative detection of Streptococcus mutans in the dental plaque of Japanese preschool children by real-time PCR.

作者信息

Hata S, Hata H, Miyasawa-Hori H, Kudo A, Mayanagi H

机构信息

Department of Oral Health Science, Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan.

出版信息

Lett Appl Microbiol. 2006 Feb;42(2):127-31. doi: 10.1111/j.1472-765X.2005.01821.x.

DOI:10.1111/j.1472-765X.2005.01821.x

PMID:16441376

Abstract

AIMS

To detect quantitatively the total bacteria and Streptococcus mutans in dental plaque by real-time PCR with prbac, Sm and GTF-B primers, and to compare their presence with the prevalence of dental caries in Japanese preschool children.

METHODS AND RESULTS

Human dental plaque samples were collected from the labial surfaces of the upper primary central incisors of 107 children. The dental status was recorded as dft by WHO caries diagnostic criteria. Positive dt and dft scores by the Sm or GTF-B primer were significantly higher than negative scores (P < 0.01). The proportions of Strep. mutans to the total bacteria from sound, and sound and/or filled upper primary incisors were significantly lower than those from decayed or filled, and decayed incisors, respectively (P < 0.01).

CONCLUSIONS

The ratios of Strep. mutans to total bacteria in plaque detected by real-time PCR with Sm and GTF-B primers were closely associated with the prevalence of dental caries in Japanese preschool children.

SIGNIFICANCE AND IMPACT OF THE STUDY

These assays may be useful for the assessment of an individual's risk of dental caries.

摘要

目的

采用prbac、Sm和GTF - B引物通过实时聚合酶链反应（PCR）定量检测牙菌斑中的总细菌和变形链球菌，并将其存在情况与日本学龄前儿童的龋齿患病率进行比较。

方法与结果

从107名儿童上颌乳中切牙唇面采集人类牙菌斑样本。根据世界卫生组织（WHO）龋齿诊断标准将牙齿状况记录为龋失补牙面数（dft）。Sm或GTF - B引物检测呈阳性的dt和dft分数显著高于阴性分数（P < 0.01）。来自健康、健康和/或已充填的上颌乳中切牙的变形链球菌占总细菌的比例分别显著低于来自龋坏或已充填、龋坏切牙的比例（P < 0.01）。