Davidovich Esti, Sarne Hadar, Shmueli Aviv, Polak David
Department of Pediatric Dentistry, Faculty of Dental Medicine, Hebrew University of Jerusalem - Hadassah Medical Center, Jerusalem, Israel.
Department of Periodontics, Faculty of Dental Medicine, Hebrew University of Jerusalem - Hadassah Medical Center, Jerusalem, Israel.
Clin Oral Investig. 2024 Oct 3;28(10):564. doi: 10.1007/s00784-024-05969-9.
This study aimed to characterize the inflammatory profile of systemically healthy children's saliva and its association with clinical diagnoses of caries and gingival inflammation.
Unstimulated saliva was collected from 100 children before clinical dental examinations. The saliva samples were analyzed for total protein and specific inflammatory cytokines (IL-10, IL-8, IL-6, and TNFα) with Bradford and ELISA assays, respectively. Salivary bacteria were quantified using a quantitative real-time polymerase chain assay. The salivary values were then correlated with age, DMFT index, plaque index (PI), and gingival index (GI).
The mean age of the cohort was 8.08 ± 0.23 years with 49% females, the mean DMF of the cohort was 2.64 ± 0.31, the mean GI was 0.51 ± 0.06, and the mean PI was 1.33 ± 0.07. Significant correlations were found between PI with DMFT and GI. Children with DMFT > 2 had significantly higher levels of IL-8 compared with children with DMFT ≤ 2. IL-6 and TNFα were significantly higher among children with PI > 1 than among children with PI ≤ 1.
Salivary cytokine were found to be associate with clinical parameters as DMFT and PI, thus may be a potential tool that reflects dental health status.
The presence of salivary cytokines in children may reflect evaluation of dental caries and oral inflammation.
本研究旨在描述全身健康儿童唾液的炎症特征及其与龋齿和牙龈炎症临床诊断的关联。
在临床口腔检查前，从100名儿童中收集未刺激唾液。分别采用Bradford法和ELISA法分析唾液样本中的总蛋白和特定炎症细胞因子（IL-10、IL-8、IL-6和TNFα）。使用定量实时聚合酶链反应测定唾液细菌数量。然后将唾液值与年龄、DMFT指数、菌斑指数（PI）和牙龈指数（GI）进行关联分析。
该队列儿童的平均年龄为8.08±0.23岁，女性占49%，队列的平均DMF为2.64±0.31，平均GI为0.51±0.06，平均PI为1.33±0.07。发现PI与DMFT和GI之间存在显著相关性。DMFT>2的儿童与DMFT≤2的儿童相比，IL-8水平显著更高。PI>1的儿童中IL-6和TNFα水平显著高于PI≤1的儿童。
发现唾液细胞因子与DMFT和PI等临床参数相关，因此可能是反映口腔健康状况的潜在工具。
儿童唾液细胞因子的存在可能反映龋齿和口腔炎症的评估情况。
