Alvarez M Lucrecia, Pinyerd Heidi L, Crisantes Jason D, Rigano M Manuela, Pinkhasov Julia, Walmsley Amanda M, Mason Hugh S, Cardineau Guy A
Center for Infectious Diseases and Vaccinology (CIDV), The Biodesign Institute at Arizona State University, 1001 South McAllister Avenue, Tempe, AZ 85287-4501, USA.
Vaccine. 2006 Mar 24;24(14):2477-90. doi: 10.1016/j.vaccine.2005.12.057. Epub 2006 Jan 13.
Yersinia pestis, the causative agent of plague, is an extremely virulent bacterium but there are no approved vaccines for protection against it. Our goal was to produce a vaccine that would address: ease of delivery, mucosal efficacy, safety, rapid scalability, and cost. We developed a novel production and delivery system for a plague vaccine of a Y. pestis F1-V antigen fusion protein expressed in tomato. Immunogenicity of the F1-V transgenic tomatoes was confirmed in mice that were primed subcutaneously with bacterially-produced F1-V and boosted orally with transgenic tomato fruit. Expression of the plague antigens in fruit allowed producing an oral vaccine candidate without protein purification and with minimal processing technology.
鼠疫耶尔森菌是鼠疫的病原体，是一种极具毒性的细菌，但目前尚无经批准用于预防鼠疫的疫苗。我们的目标是研发一种疫苗，该疫苗应具备：易于接种、黏膜有效性、安全性、快速可扩展性以及低成本等特性。我们开发了一种新型的生产和接种系统，用于生产在番茄中表达的鼠疫耶尔森菌F1-V抗原融合蛋白的鼠疫疫苗。在先用细菌生产的F1-V进行皮下初免，然后用转基因番茄果实进行口服加强免疫的小鼠中，证实了F1-V转基因番茄的免疫原性。鼠疫抗原在果实中的表达使得无需进行蛋白质纯化且采用最少的加工技术就能生产出一种口服疫苗候选物。
