Yeh Chi-Tai, Yen Gow-Chin
Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan.
J Nutr Biochem. 2006 Aug;17(8):561-9. doi: 10.1016/j.jnutbio.2005.10.008. Epub 2005 Nov 10.
Phenolic acids have significant biological and pharmacological properties and some have demonstrated remarkable ability to alter sulfate conjugation. However, the modulatory effects of phenolic acids on phenol sulfotransferases (PSTs) in vivo have not been described. The present investigation evaluates the role of phenolic acid on the expression of PSTs in male Sprague-Dawley rat liver. According to the results, gentisic acid, gallic acid and p-coumaric acid in a dosage of 100 mg/kg of body weight for 14 consecutive days significantly increased P-form PST (PST-P) activity as compared with that of the control rats (P<.05), whereas the activity of M-form PST (PST-M) in rats that received gallic acid and p-coumaric acid were also significantly (P<.05) higher than in the control rats. Reverse transcriptase-polymerase chain reaction results indicated that the changes in both PST-P and PST-M mRNA levels by phenolic acids were similar to those noted in the enzyme activity levels. The plasma obtained from phenolic acid-administered rats had significantly (P<.05) increased oxygen radical absorbance capacity (ORAC(ROO*) values than that from control rats. In a bioavailability study, following oral administration of gallic acid and p-coumaric acid, the phenolic acids were detected in the plasma, and the Cmax values after 2.0-h administration were 665+/-23 and 550+/-33 nmol/L, respectively. There was a significant correlation between the activity of both forms of PSTs and the antioxidant capacity of ORAC(ROO*) value by phenolic acids (r=.74, P<.05 and r=.77, P<.05). These data suggest that phenolic acids might alter sulfate conjugation and antioxidant capacity in living systems.
酚酸具有显著的生物学和药理学特性，一些酚酸已显示出改变硫酸结合作用的显著能力。然而，酚酸对体内酚磺基转移酶（PSTs）的调节作用尚未见报道。本研究评估了酚酸对雄性Sprague-Dawley大鼠肝脏中PSTs表达的作用。结果显示，连续14天以100 mg/kg体重的剂量给予龙胆酸、没食子酸和对香豆酸，与对照大鼠相比，显著提高了P型PST（PST-P）的活性（P<0.05），而接受没食子酸和对香豆酸的大鼠中M型PST（PST-M）的活性也显著高于对照大鼠（P<0.05）。逆转录聚合酶链反应结果表明，酚酸引起的PST-P和PST-M mRNA水平的变化与酶活性水平的变化相似。给予酚酸的大鼠血浆中的氧自由基吸收能力（ORAC(ROO*)值）显著高于对照大鼠（P<0.05）。在一项生物利用度研究中，口服没食子酸和对香豆酸后，在血浆中检测到了酚酸，给药2.0小时后的Cmax值分别为665±23和550±33 nmol/L。两种形式的PSTs活性与酚酸的ORAC(ROO*)值抗氧化能力之间存在显著相关性（r = 0.74，P<0.05和r = 0.77，P<0.05）。这些数据表明，酚酸可能会改变生物系统中的硫酸结合作用和抗氧化能力。
